Abstract

Synchronizing rhythms of behaviour and metabolic processes is important for cardiovascular health and preventing metabolic diseases. The nuclear receptors REV-ERB-α and REV-ERB-β have an integral role in regulating the expression of core clock proteins driving rhythms in activity and metabolism. Here we describe the identification of potent synthetic REV-ERB agonists with in vivo activity. Administration of synthetic REV-ERB ligands alters circadian behaviour and the circadian pattern of core clock gene expression in the hypothalami of mice. The circadian pattern of expression of an array of metabolic genes in the liver, skeletal muscle and adipose tissue was also altered, resulting in increased energy expenditure. Treatment of diet-induced obese mice with a REV-ERB agonist decreased obesity by reducing fat mass and markedly improving dyslipidaemia and hyperglycaemia. These results indicate that synthetic REV-ERB ligands that pharmacologically target the circadian rhythm may be beneficial in the treatment of sleep disorders as well as metabolic diseases.

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Acknowledgements

This work was supported by National Institutes of Health Grants (DK080201, MH092769 and DK089984) and the Howard Hughes Medical Institute. L.A.S. is the recipient of an individual National Research Service Award (DK088499).

Author information

Author notes

    • Laura A. Solt
    •  & Yongjun Wang

    These authors contributed equally to this work.

Affiliations

  1. Department of Molecular Therapeutics, The Scripps Research Institute, Jupiter, Florida 33458, USA

    • Laura A. Solt
    • , Yongjun Wang
    • , Subhashis Banerjee
    • , Travis Hughes
    • , Douglas J. Kojetin
    • , Thomas Lundasen
    • , Jin Liu
    •  & Thomas P. Burris
  2. Translational Research Institute, The Scripps Research Institute, Jupiter, Florida 33458, USA

    • Youseung Shin
    • , Michael D. Cameron
    • , Romain Noel
    •  & Theodore M. Kamenecka
  3. Howard Hughes Medical Institute and Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA

    • Seung-Hee Yoo
    •  & Joseph S. Takahashi
  4. Department of Metabolism and Aging, The Scripps Research Institute, Jupiter, Florida 33458, USA

    • Andrew A. Butler
  5. Center for Diabetes and Metabolic Diseases, The Scripps Research Institute, Jupiter, Florida 33458, USA

    • Thomas P. Burris

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Contributions

T.P.B. conceived the project. R.N., Y.S. and T.M.K. synthesized and analysed the ligands. L.A.S., Y.W., S.B., D.J.K. and T.P.B. designed/analysed and/or performed the transfection and biochemical assays. D.J.K. and T.H. designed and performed the CD analysis. T.P.B., L.A.S. and A.A.B. designed, analysed and performed the metabolic studies. Y.W., T.P.B., S.B. and T.L designed/analysed and/or performed the circadian gene expression and behaviour analysis. J.L. performed gene expression analysis. S.-H.Y. and J.S.T. designed and performed the studies using the Per2-luc mouse tissues. M.D.C. performed the pharmacokinetic analysis. T.P.B. wrote the manuscript with input from all of the authors.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Thomas P. Burris.

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    Supplementary Information

    This file contains Supplementary Methods with additional references and Supplementary Figures 1–15.

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DOI

https://doi.org/10.1038/nature11030

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