Letter | Published:

Electron tomography at 2.4-ångström resolution

Nature volume 483, pages 444447 (22 March 2012) | Download Citation

Abstract

Transmission electron microscopy is a powerful imaging tool that has found broad application in materials science, nanoscience and biology1,2,3. With the introduction of aberration-corrected electron lenses, both the spatial resolution and the image quality in transmission electron microscopy have been significantly improved4,5 and resolution below 0.5 ångströms has been demonstrated6. To reveal the three-dimensional (3D) structure of thin samples, electron tomography is the method of choice7,8,9,10,11, with cubic-nanometre resolution currently achievable10,11. Discrete tomography has recently been used to generate a 3D atomic reconstruction of a silver nanoparticle two to three nanometres in diameter12, but this statistical method assumes prior knowledge of the particle’s lattice structure and requires that the atoms fit rigidly on that lattice. Here we report the experimental demonstration of a general electron tomography method that achieves atomic-scale resolution without initial assumptions about the sample structure. By combining a novel projection alignment and tomographic reconstruction method with scanning transmission electron microscopy, we have determined the 3D structure of an approximately ten-nanometre gold nanoparticle at 2.4-ångström resolution. Although we cannot definitively locate all of the atoms inside the nanoparticle, individual atoms are observed in some regions of the particle and several grains are identified in three dimensions. The 3D surface morphology and internal lattice structure revealed are consistent with a distorted icosahedral multiply twinned particle. We anticipate that this general method can be applied not only to determine the 3D structure of nanomaterials at atomic-scale resolution13,14,15, but also to improve the spatial resolution and image quality in other tomography fields7,9,16,17,18,19,20.

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Acknowledgements

We thank E. J. Kirkland for help with multislice STEM calculations, R. F. Egerton, Z. H. Zhou and J. A. Rodríguez for discussions and I. Atanasov for assistance in data acquisition. The tomographic tilt series were acquired at the Electron Imaging Center for NanoMachines of the California NanoSystems Institute. This work was partially supported by UC Discovery/TomoSoft Technologies (IT107-10166).

Author information

Author notes

    • M. C. Scott
    • , Chien-Chun Chen
    •  & Matthew Mecklenburg

    These authors contributed equally to this work.

Affiliations

  1. Department of Physics and Astronomy and California NanoSystems Institute, University of California, Los Angeles, California 90095, USA

    • M. C. Scott
    • , Chien-Chun Chen
    • , Matthew Mecklenburg
    • , Chun Zhu
    • , Rui Xu
    • , B. C. Regan
    •  & Jianwei Miao
  2. National Center for Electron Microscopy, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA

    • Peter Ercius
    •  & Ulrich Dahmen

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Contributions

J.M. conceived the overall project; M.C.S., M.M., C.Z., B.C.R. and J.M. designed and conducted the experiments; C.Z., R.X., C.-C.C., P.E. and J.M. did multislice STEM calculations; C.-C.C. and J.M. performed the data analysis and image reconstruction; U.D., J.M., C.-C.C., M.C.S., M.M. and B.C.R interpreted the results, and J.M., M.C.S., C.-C.C. and M.M. wrote the manuscript. All authors commented on the manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Jianwei Miao.

Supplementary information

PDF files

  1. 1.

    Supplementary Information

    This file contains Supplementary Methods, Supplementary Figures 1-13 and Supplementary Table 1.

Videos

  1. 1.

    Supplementary Movie 1

    This movie shows a 3D volume rendering of the reconstructed gold nanoparticle.

  2. 2.

    Supplementary Movie 2

    This movie shows a 3D iso-surface rendering of the reconstructed gold nanoparticle.

  3. 3.

    Supplementary Movie 3

    This movie shows a 3D volume rendering of the four major grains determined from the reconstructed gold nanoparticle.

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DOI

https://doi.org/10.1038/nature10934

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