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Regulation of Caenorhabditis elegans lifespan by sir-2.1 transgenes


Arising from H. A. Tissenbaum & L. Guarente Nature 410, 227–230 (2001)10.1038/35065638

Tissenbaum and Guarente1 identified the first metazoan Sir2 homologue shown to affect lifespan, Caenorhabditis elegans sir-2.1. Independent transgenic lines harbouring extrachromosomal DNA arrays containing sir-2.1 and the dominant transgene marker rol-6(su1006) were reported to extend mean lifespan between 15% and 50%1. Similar extensions in mean lifespan were also found for lines in which the sir-2.1 transgenic arrays were integrated into the genome following γ-irradiation1. However, the extension of lifespan was overestimated in a high-copy sir-2.1 transgene-containing worm strain because of an unlinked mutation.

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Figure 1: Integrated transgene geIs3 , expressing sir-2.1 from its intergenic promoter (P int ), extends C. elegans lifespan following outcross of a linked dye-filling mutation.


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M.V. and L.P.G. planned experiments. M.V. performed experiments and analysed data.

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Correspondence to Leonard Guarente.

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Competing financial interests: LG is co-chair of the SAB of Sirtris/GSK.

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Viswanathan, M., Guarente, L. Regulation of Caenorhabditis elegans lifespan by sir-2.1 transgenes. Nature 477, E1–E2 (2011).

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