Abstract
Arising from: Z. Shcheprova, S. Baldi, S. B. Frei, G. Gonnet & Y. Barral Nature 454, 728–734 (2008)10.1038/nature07212
During mitosis in Saccharomyces cerevisiae, senescence factors such as extrachromosomal ribosomal DNA circles (ERCs) are retained in the mother cell and excluded from the bud/daughter cell1. Shcheprova et al.2 proposed a model suggesting segregation of ERCs through their association with nuclear pore complexes (NPCs) and retention of pre-existing NPCs in the mother cell during mitosis. However, this model is inconsistent with previous data3 and we demonstrate here that NPCs do efficiently migrate from the mother into the bud. Therefore, binding to NPCs does not seem to explain the retention of ERCs in the mother cell.
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On the basis of photobleaching experiments, Shcheprova et al.2 concluded that NPCs are prevented from migrating through the bud neck in anaphase and that new NPCs are inserted into the bud (but not the mother) nuclear envelope. Two testable predictions arise from this model: first, the number of NPCs in the mother nuclear envelope should remain constant throughout anaphase; and second, the total number of NPCs in the nuclear envelope should increase abruptly during anaphase.
Using fluorescence time-lapse microscopy, we found that the intensity of GFP-tagged core NPC components at the nuclear envelope increased continuously during G1 phase, S phase and early mitosis but remained constant during nuclear migration through the bud neck in anaphase. Upon movement of the nucleus into the bud, the appearance of the GFP signal in the bud was associated with a pronounced GFP intensity drop in the mother (Fig. 1a, b). These results contradict the predictions of the model of Shcheprova et al.2.
A serial thin-sectioning electron microscopy study3, not cited by Shcheprova et al.2, showed that the number of NPCs in the nuclear envelope increases continuously from G1 (86 ± 16) to S phase (117 ± 17) to early mitosis (142 ± 17) and remains constant during anaphase (137 ± 29) (Table 1 in ref. 3). These results are incompatible with the model of Shcheprova et al.2 but consistent with our data (Fig. 1a, b).
Shcheprova et al.2 investigated NPC movement between mother and bud using fluorescence loss in photobleaching (FLIP) experiments with GFP-tagged NPC components. Repeated photobleaching of GFP in the mother nuclear envelope induced a decrease of the nuclear envelope fluorescence in the mother whereas the signal in the bud remained constant (Fig. 1a, b in ref. 2). Shcheprova et al.2 assumed that the signal in the bud was not affected by photobleaching and concluded that NPCs do not move from the mother to the bud during anaphase. However, Fig. 1a, b shows that the signal in the bud strongly increases during anaphase. If the GFP signal of bud NPCs was independent of the pre-existing pores in the mother cell, the FLIP experiments should have shown an increase in the bud signal; instead Shcheprova et al.2 reported a constant signal. This inconsistency, which would have become apparent if the FLIP experiments had been conducted with respect to unbleached control cells, suggests that substantial numbers of NPCs migrate from the mother into the bud during anaphase.
To investigate this, we used strains expressing NPC components fused to the photoconvertible fluorescent protein Dendra24. Upon photoconversion in the mother, movement of marked (red-fluorescent) NPCs to the bud could be observed (Fig. 1c; see also http://www.embl.de/SegregationMovie for a movie of this photoconversion experiment; movie also available from the authors). Analogous results were also obtained using iFRAP (see Appendix).
Photoconversion of all NPCs at the nuclear envelope in metaphase (using Nup49 or Nic96 tagged with Dendra2) and quantification of the red fluorescence in the two resulting nuclei after cell division indicated that 38 ± 12% (mean ± s.d., n = 50) of pre-existing NPCs moved into the bud during mitosis. The surface density of marked NPCs is roughly equal in both compartments, as the surface area of the bud nucleus is only ∼70% of the mother nucleus at the end of telophase (as determined by quantification of the NLS–GFP intensity in mother and bud nuclei in a separate experiment (n = 93), consistent with a previous report5). We conclude that NPCs effectively migrate into the bud along with the nucleus.
Our results and those of ref. 3 demonstrate that pre-existing NPCs are not retained in the mother but are partitioned between mother and bud nuclei proportionally to nuclear surface area. This questions the model for asymmetric segregation of ERCs during yeast mitosis based on the general retention of pre-existing NPCs in the mother, as proposed by Shcheprova et al.2 and highlighted elsewhere6,7,8,9,10. Therefore, how asymmetric segregation of ERCs is achieved remains an open question.
APPENDIX
An inverse fluorescence recovery after photobleaching (iFRAP) experiment demonstrates the appearance of NPC signal in the bud in the absence of a cytoplasmic labelled protein reservoir (Appendix Fig. 1a; quantification in Appendix Fig. 1b).
Methods
NPC components were chromosomally tagged at the carboxy terminus using homologous recombination.
Imaging was performed on a Deltavision RT widefield microscope (Fig. 1) or a Leica SP-2 confocal microscope (see Appendix). For photoconversion, a 405-nm laser was used with a diffraction-limited spot, either on a Deltavision RT (Fig. 1c) or a Perkin Elmer Ultraview VoX spinning disc microscope (the photoconversion experiment quoted in the text). Flat-field, photobleaching and autofluorescence corrections were applied to all images.
References
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Khmelinskii, A., Keller, P., Lorenz, H. et al. Segregation of yeast nuclear pores. Nature 466, E1 (2010). https://doi.org/10.1038/nature09255
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DOI: https://doi.org/10.1038/nature09255
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