Despite our rapidly growing knowledge about the human genome, we do not know all of the genes required for some of the most basic functions of life. To start to fill this gap we developed a high-throughput phenotypic screening platform combining potent gene silencing by RNA interference, time-lapse microscopy and computational image processing. We carried out a genome-wide phenotypic profiling of each of the ∼21,000 human protein-coding genes by two-day live imaging of fluorescently labelled chromosomes. Phenotypes were scored quantitatively by computational image processing, which allowed us to identify hundreds of human genes involved in diverse biological functions including cell division, migration and survival. As part of the Mitocheck consortium, this study provides an in-depth analysis of cell division phenotypes and makes the entire high-content data set available as a resource to the community.
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We thank J. Gagneur for suggestions on data processing; S. Berthoumieux for assistance in computation; Y. Sun for coordination support in Mitocheck; U. Ringeisen for help in preparing the figures; S. Winkler and L. Burger and EMBL’s electronic and mechanical workshops for support in microscope development; Olympus Soft Imaging Solutions (OSIS) and Olympus Europe for collaboration; Leica Microsystems for collaboration; Applied Biosystems for providing unpublished validation data of the siRNA library; and all our colleagues in the Mitocheck consortium for collaboration. This project was funded by grants to J.E. (within the Mitocheck consortium by the European Commission (LSHG-CT-2004-503464) as well as by the Federal Ministry of Education and Research (BMBF) in the framework of the National Genome Research Network (NGFN) (NGFN-2 SMP-RNAi, FKZ01GR0403)), to R.P. (BMBF NGFN2 SMP-Cell, FKZ01GR0423) as well as to J.E. and R.P. by the Landesstiftung Baden Wuerttemberg in the framework of the research programme ‘RNS/RNAi’. R.D. is supported by the Wellcome Trust.
Author Contributions B.N. developed the primary screen assay. P.R., H.E., B.N. and J.B. generated the data for the primary and validation screen. T.W. and M.H. developed the image processing software. T.W., J.-K.H., G.P., and W.H. analysed the data. J.-K.H., V.S. and R.S. performed the bioinformatics analysis. B.N., P.R., J.B., T.W. and C.Co. conducted the quality control and manual annotation. U.L., C.Co., F.S. and R.P. developed the HT microscope platform. H.E. and R.P. developed the HT transfection platform. J.-K.H. and R.D. created the Mitocheck database and website. C.Ce. and R.P. designed the manual annotation database. J.B., C.Co., B.N. and A.W. created the data for the spindle assay. R.K. and R.E. provided IT support. I.P. and A.A.H. provided the BAC cell pools. M.H.A.S., C.Ch. and D.W.G. provided reagents. J.-M.P. coordinated the Mitocheck consortium. J.E. coordinated and supervised the project and wrote the manuscript.
The authors declare no competing financial interests.
This file contains Supplementary Methods, Supplementary References, Supplementary Figures 1-11 with legends and Legends for Supplementary Movies 1-14. (PDF 2252 kb)
This zipped file contains Supplementary Tables 1-7. (ZIP 580 kb)
This zipped file contains Supplementary Movies 1-15 which show an overview of the representative morphology for each of the sixteen classes shown in Figure 1b. (ZIP 32444 kb)
This zipped file contains Supplementary Movies 16-30 which show the highlighted single cell from movie 1-15 (Figure 1b). (ZIP 1991 kb)
This zipped file contains Supplementary Movies 31-40 which show the spindle morphology phenotypes represented in Figure 5. (ZIP 26408 kb)
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Neumann, B., Walter, T., Hériché, JK. et al. Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes. Nature 464, 721–727 (2010). https://doi.org/10.1038/nature08869
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