Letter | Published:

MONOPTEROS controls embryonic root initiation by regulating a mobile transcription factor

Nature volume 464, pages 913916 (08 April 2010) | Download Citation

Abstract

Acquisition of cell identity in plants relies strongly on positional information1, hence cell–cell communication and inductive signalling are instrumental for developmental patterning. During Arabidopsis embryogenesis, an extra-embryonic cell is specified to become the founder cell of the primary root meristem, hypophysis, in response to signals from adjacent embryonic cells2. The auxin-dependent transcription factor MONOPTEROS (MP) drives hypophysis specification by promoting transport of the hormone auxin from the embryo to the hypophysis precursor. However, auxin accumulation is not sufficient for hypophysis specification, indicating that additional MP-dependent signals are required3. Here we describe the microarray-based isolation of MP target genes that mediate signalling from embryo to hypophysis. Of three direct transcriptional target genes, TARGET OF MP 5 (TMO5) and TMO7 encode basic helix–loop–helix (bHLH) transcription factors that are expressed in the hypophysis-adjacent embryo cells, and are required and partially sufficient for MP-dependent root initiation. Importantly, the small TMO7 transcription factor moves from its site of synthesis in the embryo to the hypophysis precursor, thus representing a novel MP-dependent intercellular signal in embryonic root specification.

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Accessions

Primary accessions

Gene Expression Omnibus

Data deposits

The microarray dataset is deposited in the Gene Expression Omnibus (GEO) with accession number GSE13881.

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Acknowledgements

We acknowledge P. van Oorschot, A. van Haperen and S. Heilbronner for technical assistance, C. Arsene for plant care, S. de Folter and O. Mathieu for advice on ChIP, M. Wunderlich and G. Bijl for help with qPCR, G. Hooiveld for help with statistical analysis of the microarray data and the Nottingham Arabidopsis Stock Centre for mutant and transgenic seeds. We thank P. Maier, S. de Vries, A. Koltunow, A. Lokerse, S. Saiga, V. Willemsen and M. Tsiantis for discussions and comments on the manuscript. This work was supported by grants from the Netherlands Organization for Scientific Research (NWO; VIDI 864.06.012 to D.W.), from the Deutsche Forschungsgemeinschaft (DFG; SFB446 to G.J.) and the Netherlands Proteomics Centre (NPC; D.W., grant awarded to S. de Vries).

Author Contributions A.S. established conditions for the microarray experiment, which was performed together with M.S., validated the array data by qPCR, generated most transgenic lines for expression analysis and functional studies of TMO genes, which was done together with B.M. M.K. performed in situ hybridizations, J.F. and D.W. performed ChIP, and W.L. generated and analysed TMO7 downregulation and TMO7-LIKE1 reporter lines. B.M. performed MP overexpression, and suspensor-specific TMO7 expression experiments, and generated pMP–GFP lines. E.H.R. identified the suspensor-specific At1g34170 gene, and generated pSUSP-GFP lines. D.W. and G.J. conceived and supervised the study. D.W. wrote the paper with input from G.J., A.S. and B.M.

Author information

Author notes

    • Alexandra Schlereth

    Present address: Syngenta Crop Protection, CH-4332 Stein, Switzerland.

Affiliations

  1. Entwicklungsgenetik, Zentrum für Molekularbiologie der Pflanzen (ZMBP), Universität Tübingen, Auf der Morgenstelle 3, 72076 Tübingen, Germany

    • Alexandra Schlereth
    • , Marika Kientz
    • , Gerd Jürgens
    •  & Dolf Weijers
  2. Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, the Netherlands

    • Barbara Möller
    • , Weilin Liu
    • , Jacky Flipse
    • , Eike H. Rademacher
    •  & Dolf Weijers
  3. Max Planck Institute for Developmental Biology, Department of Molecular Biology, Spemannstrasse 37–39, 72076 Tübingen, Germany

    • Markus Schmid

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Correspondence to Gerd Jürgens or Dolf Weijers.

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    Supplementary Information

    This file contains Supplementary Figures S1-S7 with legends, and Supplementary Tables S1-S3.

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DOI

https://doi.org/10.1038/nature08836

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