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Mical links semaphorins to F-actin disassembly

Abstract

How instructive cues present on the cell surface have their precise effects on the actin cytoskeleton is poorly understood. Semaphorins are one of the largest families of these instructive cues and are widely studied for their effects on cell movement, navigation, angiogenesis, immunology and cancer1. Semaphorins/collapsins were characterized in part on the basis of their ability to drastically alter actin cytoskeletal dynamics in neuronal processes2, but despite considerable progress in the identification of semaphorin receptors and their signalling pathways3, the molecules linking them to the precise control of cytoskeletal elements remain unknown. Recently, highly unusual proteins of the Mical family of enzymes have been found to associate with the cytoplasmic portion of plexins, which are large cell-surface semaphorin receptors, and to mediate axon guidance, synaptogenesis, dendritic pruning and other cell morphological changes4,5,6,7. Mical enzymes perform reduction–oxidation (redox) enzymatic reactions4,5,8,9,10 and also contain domains found in proteins that regulate cell morphology4,11. However, nothing is known of the role of Mical or its redox activity in mediating morphological changes. Here we report that Mical directly links semaphorins and their plexin receptors to the precise control of actin filament (F-actin) dynamics. We found that Mical is both necessary and sufficient for semaphorin–plexin-mediated F-actin reorganization in vivo. Likewise, we purified Mical protein and found that it directly binds F-actin and disassembles both individual and bundled actin filaments. We also found that Mical utilizes its redox activity to alter F-actin dynamics in vivo and in vitro, indicating a previously unknown role for specific redox signalling events in actin cytoskeletal regulation. Mical therefore is a novel F-actin-disassembly factor that provides a molecular conduit through which actin reorganization—a hallmark of cell morphological changes including axon navigation—can be precisely achieved spatiotemporally in response to semaphorins.

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Figure 1: Mical regulates actin-rich cellular process morphology.
Figure 2: Semaphorin, plexin and Mical control F-actin organization and bundling.
Figure 3: Semaphorin–plexin-mediated actin rearrangements require Mical, which binds and directly regulates actin dynamics.
Figure 4: Mical directly disassembles F-actin and regulates growth cone morphology.

References

  1. Tran, T. S., Kolodkin, A. L. & Bharadwaj, R. Semaphorin regulation of cellular morphology. Annu. Rev. Cell Dev. Biol. 23, 263–292 (2007)

    CAS  Article  Google Scholar 

  2. Luo, Y., Raible, D. & Raper, J. A. Collapsin: a protein in brain that induces the collapse and paralysis of neuronal growth cones. Cell 75, 217–227 (1993)

    CAS  Article  Google Scholar 

  3. Zhou, Y., Gunput, R. A. & Pasterkamp, R. J. Semaphorin signaling: progress made and promises ahead. Trends Biochem. Sci. 33, 161–170 (2008)

    CAS  Article  Google Scholar 

  4. Terman, J. R., Mao, T., Pasterkamp, R. J., Yu, H. H. & Kolodkin, A. L. MICALs, a family of conserved flavoprotein oxidoreductases, function in plexin-mediated axonal repulsion. Cell 109, 887–900 (2002)

    CAS  Article  Google Scholar 

  5. Schmidt, E. F., Shim, S. O. & Strittmatter, S. M. Release of MICAL autoinhibition by semaphorin-plexin signaling promotes interaction with collapsin response mediator protein. J. Neurosci. 28, 2287–2297 (2008)

    CAS  Article  Google Scholar 

  6. Beuchle, D., Schwarz, H., Langegger, M., Koch, I. & Aberle, H. Drosophila MICAL regulates myofilament organization and synaptic structure. Mech. Dev. 124, 390–406 (2007)

    CAS  Article  Google Scholar 

  7. Kirilly, D. et al. A genetic pathway composed of Sox14 and Mical governs severing of dendrites during pruning. Nature Neurosci. 12, 1497–1505 (2009)

    CAS  Article  Google Scholar 

  8. Siebold, C. et al. High-resolution structure of the catalytic region of MICAL (molecule interacting with CasL), a multidomain flavoenzyme-signaling molecule. Proc. Natl Acad. Sci. USA 102, 16836–16841 (2005)

    ADS  CAS  Article  Google Scholar 

  9. Nadella, M., Bianchet, M. A., Gabelli, S. B., Barrila, J. & Amzel, L. M. Structure and activity of the axon guidance protein MICAL. Proc. Natl Acad. Sci. USA 102, 16830–16835 (2005)

    ADS  CAS  Article  Google Scholar 

  10. Gupta, N. & Terman, J. R. in Flavins and Flavoproteins 2008 (eds Frago, S., Gómez-Moreno, C. & Medina, M.) 345–350 (Proc. 16th Int. Symp. Flavins Flavoproteins, Prensas Univ. Zaragoza, 2008)

    Google Scholar 

  11. Suzuki, T. et al. MICAL, a novel CasL interacting molecule, associates with vimentin. J. Biol. Chem. 277, 14933–14941 (2002)

    CAS  Article  Google Scholar 

  12. Tilney, L. G. & DeRosier, D. J. How to make a curved Drosophila bristle using straight actin bundles. Proc. Natl Acad. Sci. USA 102, 18785–18792 (2005)

    ADS  CAS  Article  Google Scholar 

  13. Sutherland, J. D. & Witke, W. Molecular genetic approaches to understanding the actin cytoskeleton. Curr. Opin. Cell Biol. 11, 142–151 (1999)

    CAS  Article  Google Scholar 

  14. Turner, C. M. & Adler, P. N. Distinct roles for the actin and microtubule cytoskeletons in the morphogenesis of epidermal hairs during wing development in Drosophila . Mech. Dev. 70, 181–192 (1998)

    CAS  Article  Google Scholar 

  15. Tilney, L. G., Connelly, P. S., Vranich, K. A., Shaw, M. K. & Guild, G. M. Actin filaments and microtubules play different roles during bristle elongation in Drosophila . J. Cell Sci. 113, 1255–1265 (2000)

    CAS  PubMed  Google Scholar 

  16. Guild, G. M., Connelly, P. S., Vranich, K. A., Shaw, M. K. & Tilney, L. G. Actin filament turnover removes bundles from Drosophila bristle cells. J. Cell Sci. 115, 641–653 (2002)

    CAS  PubMed  Google Scholar 

  17. Geng, W., He, B., Wang, M. & Adler, P. N. The tricornered gene, which is required for the integrity of epidermal cell extensions, encodes the Drosophila nuclear DBF2-related kinase. Genetics 156, 1817–1828 (2000)

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Fan, J. et al. The organization of F-actin and microtubules in growth cones exposed to a brain-derived collapsing factor. J. Cell Biol. 121, 867–878 (1993)

    ADS  CAS  Article  Google Scholar 

  19. Sánchez-Soriano, N. & Prokop, A. The influence of pioneer neurons on a growing motor nerve in Drosophila requires the neural cell adhesion molecule homolog FasciclinII. J. Neurosci. 25, 78–87 (2005)

    Article  Google Scholar 

  20. Fan, J. & Raper, J. A. Localized collapsing cues can steer growth cones without inducing their full collapse. Neuron 14, 263–274 (1995)

    CAS  Article  Google Scholar 

  21. Dent, E. W., Barnes, A. M., Tang, F. & Kalil, K. Netrin-1 and semaphorin 3A promote or inhibit cortical axon branching, respectively, by reorganization of the cytoskeleton. J. Neurosci. 24, 3002–3012 (2004)

    CAS  Article  Google Scholar 

  22. Kapfhammer, J. P. & Raper, J. A. Collapse of growth cone structure on contact with specific neurites in culture. J. Neurosci. 7, 201–212 (1987)

    CAS  Article  Google Scholar 

  23. Campbell, D. S. et al. Semaphorin 3A elicits stage-dependent collapse, turning, and branching in Xenopus retinal growth cones. J. Neurosci. 21, 8538–8547 (2001)

    CAS  Article  Google Scholar 

  24. Fenstermaker, V., Chen, Y., Ghosh, A. & Yuste, R. Regulation of dendritic length and branching by semaphorin 3A. J. Neurobiol. 58, 403–412 (2004)

    CAS  Article  Google Scholar 

  25. Liu, Y. & Halloran, M. C. Central and peripheral axon branches from one neuron are guided differentially by Semaphorin3D and transient axonal glycoprotein-1. J. Neurosci. 25, 10556–10563 (2005)

    CAS  Article  Google Scholar 

  26. Sakai, J. A. & Halloran, M. C. Semaphorin 3d guides laterality of retinal ganglion cell projections in zebrafish. Development 133, 1035–1044 (2006)

    CAS  Article  Google Scholar 

  27. Tosney, K. W. & Landmesser, L. T. Growth cone morphology and trajectory in the lumbosacral region of the chick embryo. J. Neurosci. 5, 2345–2358 (1985)

    CAS  Article  Google Scholar 

  28. Broadie, K. et al. From growth cone to synapse: the life history of the RP3 motor neuron. Dev. Suppl. 227–238 (1993)

  29. Godement, P., Wang, L. C. & Mason, C. A. Retinal axon divergence in the optic chiasm: dynamics of growth cone behavior at the midline. J. Neurosci. 14, 7024–7039 (1994)

    CAS  Article  Google Scholar 

  30. Murray, M. J., Merritt, D. J., Brand, A. H. & Whitington, P. M. In vivo dynamics of axon pathfinding in the Drosophila CNS: a time-lapse study of an identified motorneuron. J. Neurobiol. 37, 607–621 (1998)

    CAS  Article  Google Scholar 

Download references

Acknowledgements

We thank C. Cowan, M. Rosen and T. Südhof for comments on drafts of our manuscript and M. Bailey, C. Gilpin, H. He, T. Januszewski, H. Krämer, W. Lin, T. Wedgeworth, X. Zhang and the University of Texas Southwestern Electron Microscopy Core Facility for discussions and assistance. We also thank the Bloomington, Harvard and Japanese stock centres for flies and H. Aberle, L. Cooley, C. Goodman, A. Kolodkin, B. Lee, L. Luo, J. Merriam and X. Zhang for flies and/or reagents. This work was supported by the US National Institute of Mental Health (MH085923) and a Basil O’Connor Starter Scholar Research Award to J.R.T. J.R.T. is a Klingenstein Fellow and the Rita C. and William P. Clements, Jr, Scholar in Medical Research.

Author Contributions All authors contributed critical reagents, discussed the results and commented on the manuscript; R.-J.H., U.Y., J.Y., H.W., T.Y. and J.R.T. performed experiments, collected and analysed data, and prepared the manuscript; and J.R.T. oversaw all aspects of the project and wrote the paper.

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Correspondence to Jonathan R. Terman.

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Hung, RJ., Yazdani, U., Yoon, J. et al. Mical links semaphorins to F-actin disassembly. Nature 463, 823–827 (2010). https://doi.org/10.1038/nature08724

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