Figure 3 : Reverse transcription and integration of BDV RNA in mammalian cells.
From: Endogenous non-retroviral RNA virus elements in mammalian genomes
a, PCR amplification of BDV N-specific cDNA in BDV-infected cells. OL and 293T, human; Vero, monkey; C6, rat; MDCK, dog. b, Schematic representation of the bornavirus genome and mRNAs. Regions for the PCR amplification are indicated by red bars. c, Region-dependent amplification of BDV cDNA in infected OL cells. The numbers on the left side of the panels correspond to the amplification regions in panel b. Ctrl indicates the results of RT–PCR using RNA from BDV-infected OL cells. d, Integration of BDV DNA. Genomic DNA was isolated from BDV-infected OL cells at the indicated days after infection, and Alu-PCR was performed with (+) or without (–) the Alu primer. UI, genomic DNA from uninfected cells; d.p.i., days post-infection.
