a, Alignment between predicted amino acid sequences of BDV N and two human bornavirus N-like elements. Black arrowheads indicate predicted frameshift sites in LOC55096. b, Sequence alignments of transcription signal sites (S1/S2 and T1) at both the 5′ and 3′ ends of the bornavirus N ORF. A schematic representation of bornavirus genome structure is shown. c, d, Low-stringency Southern blot hybridizations of DNA from various mammalian species using human EBLN-1 and mouse EBLN chr.11 (c) and TLS EBLN (d) as probes. Dots and arrowheads on the right side of the murine and shrew lanes in panel c indicate the positions of reproducible positive signals. Mr, Molecular marker.