What recent ribosome structures have revealed about the mechanism of translation

Abstract

The high-resolution structures of ribosomal subunits published in 2000 have revolutionized the field of protein translation. They facilitated the determination and interpretation of functional complexes of the ribosome by crystallography and electron microscopy. Knowledge of the precise positions of residues in the ribosome in various states has facilitated increasingly sophisticated biochemical and genetic experiments, as well as the use of new methods such as single-molecule kinetics. In this review, we discuss how the interaction between structural and functional studies over the last decade has led to a deeper understanding of the complex mechanisms underlying translation.

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Figure 1: Structure of the ribosome.
Figure 2: Overview of bacterial translation.
Figure 3: Decoding by the ribosome.
Figure 4: Peptide-bond formation.
Figure 5: EF-G catalysed translocation.
Figure 6: Termination of translation by class I release factors.

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Acknowledgements

We thank R. Voorhees for a critical reading of this manuscript, and J. Frank and X. Aggirrezabala for providing coordinates of a hybrid state complex. Work in V.R.’s laboratory is supported by the Medical Research Council (UK), the Wellcome Trust, the Louis-Jeantet Foundation and the Agouron Institute. T.M.S. was supported by fellowships from the Human Frontiers Science Program and Emmanuel College, Cambridge. Part of this review was written when V.R. was a G. N. Ramachandran Visiting Professor at the Indian Institute of Science, Bangalore, where he thanks U. Varshney for his hospitality and useful discussions.

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Correspondence to T. Martin Schmeing or V. Ramakrishnan.

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Schmeing, T., Ramakrishnan, V. What recent ribosome structures have revealed about the mechanism of translation. Nature 461, 1234–1242 (2009) doi:10.1038/nature08403

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