Figure 2: Quantitative and correlative analysis of keratocyte morphology and speed. | Nature

Figure 2: Quantitative and correlative analysis of keratocyte morphology and speed.

From: Mechanism of shape determination in motile cells

Figure 2

a, The distributions of measures across a population of live keratocytes (left panels) are contrasted with values through time for 11 individual cells (right). Within each histogram, the population mean ± one standard deviation is shown by the left vertical bar, whereas the population mean ± the average standard deviation exhibited by individual cells over 5 min is shown by the right bar. b, Significant pair-wise correlations (P < 0.05; bootstrap confidence intervals) within a population of keratocytes are diagrammed (left panel). Two additional measures are included: front roughness, which measures the local irregularity of the leading edge, and actin ratio, which represents the peakedness of the actin distribution along the leading edge. The correlations indicate that, apart from size differences, cells lie along a single phenotypic continuum (right panel), from ‘decoherent’ to ‘coherent’. Decoherent cells move slowly and assume rounded shapes with low aspect ratios and high lamellipodial curvatures. The actin network is less ordered, with ragged leading edges and low actin ratios. Coherent cells move faster and have lower lamellipodial curvature. The actin network is highly ordered with smooth leading edges and high actin ratios. c, Phase-contrast images depict a cell transiently treated with DMSO (Supplementary Movie 1), which caused a reversible inhibition of motility and loss of the lamellipodium. Images shown correspond to before (20 s), during (610 s) and two time points after (830 s and 1,230 s) the perturbation. d, Time traces of area, aspect ratio and speed for the cell in c show that shape and speed are regained post perturbation. Dashed lines show time points from c; arrowheads indicate the time of perturbation. e, Area, aspect ratio and speed of nine cells are shown as averages obtained from one-minute windows before, during and after DMSO treatment (shown sequentially from left to right for each cell). The cell shown in c and d is highlighted.

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