Localization and functionality of microsporidian iron–sulphur cluster assembly proteins

Abstract

Microsporidia are highly specialized obligate intracellular parasites of other eukaryotes (including humans1) that show extreme reduction at the molecular, cellular and biochemical level2,3. Although microsporidia have long been considered as early branching eukaryotes that lack mitochondria4, they have recently been shown to contain a tiny mitochondrial remnant called a mitosome2,5. The function of the mitosome is unknown, because microsporidians lack the genes for canonical mitochondrial functions, such as aerobic respiration and haem biosynthesis. However, microsporidial genomes encode several components of the mitochondrial iron–sulphur (Fe–S) cluster assembly machinery. Here we provide experimental insights into the metabolic function and localization of these proteins. We cloned, functionally characterized and localized homologues of several central mitochondrial Fe–S cluster assembly components for the microsporidians Encephalitozoon cuniculi and Trachipleistophora hominis. Several microsporidial proteins can functionally replace their yeast counterparts in Fe–S protein biogenesis. In E. cuniculi, the iron (frataxin) and sulphur (cysteine desulphurase, Nfs1) donors and the scaffold protein (Isu1) co-localize with mitochondrial Hsp70 to the mitosome, consistent with it being the functional site for Fe–S cluster biosynthesis. In T. hominis, mitochondrial Hsp70 and the essential sulphur donor (Nfs1) are still in the mitosome, but surprisingly the main pools of Isu1 and frataxin are cytosolic, creating a conundrum of how these key components of Fe–S cluster biosynthesis coordinate their function. Together, our studies identify the essential biosynthetic process of Fe–S protein assembly as a key function of microsporidian mitosomes.

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Figure 1: Trachipleistophora hominis Fe–S cluster proteins are functional in Fe–S cluster biosynthesis reactions.
Figure 2: Mitochondrial-targeted EcYfh1 and EcGrx5 (partially) restore Fe–S protein maturation in Yfh1- and Grx5-depleted yeast cells.
Figure 3: Cellular localization of Isu1-, Yfh1- and Nfs1-like proteins in E. cuniculi by immunofluorescence and confocal microscopy.
Figure 4: Cellular localization of Isu1-, Yfh1- and Nfs1-like proteins in T. hominis.

Accession codes

Primary accessions

GenBank/EMBL/DDBJ

Data deposits

The new T. hominis sequences have been deposited in GenBank under accession numbers EF571313EF571315 and EU282037.

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Acknowledgements

A.V.G. acknowledges the support of a Marie Curie Fellowship from the European Commission and T.M.E. acknowledges support from the British Royal Society and the Leverhulme Trust. We thank A. J. Pierik and C. Noel for help in identifying ThIsd11;, J. Ihrig and B. Keys for experimental support; and T. Booth for help with confocal microscopy. R.L. acknowledges support from Deutsche Forschungsgemeinschaft (Gottfried-Wilhelm Leibniz program and SFB-TR1), European Commission (MitEURO), and Fonds der Chemischen Industrie.

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Correspondence to Roland Lill or T. Martin Embley.

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Goldberg, A., Molik, S., Tsaousis, A. et al. Localization and functionality of microsporidian iron–sulphur cluster assembly proteins. Nature 452, 624–628 (2008). https://doi.org/10.1038/nature06606

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