Abstract
Arising from: J. A. Byrne et al. Nature 450, doi: 10.1038/nature06357 (2007)
Somatic cell nuclear transfer (SCNT) into enucleated oocytes has emerged as a technique that can be used to derive mouse embryonic stem cell lines with defined genotypes. In this issue Byrne et al.1 report the derivation of two SCNT Rhesus macaca male stem cell lines designated CRES-1 and CRES-2. Molecular studies detailed in their paper provides supporting evidence that the chromosome complement of CRES-1 and CRES-2 was genetically identical to the male cell donor nucleus and that the mitochondrial DNA originated from different recipient oocytes. In this validation paper, we independently confirm that both stem cell lines were indeed derived by SCNT.
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References
Byrne, J. A. et al. Producing primate embryonic stem cells by somatic cell nuclear transfer. Nature (this issue). 10.1038/nature06357 (2007)
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Cram, D., Song, B. & Trounson, A. Genotyping of Rhesus SCNT pluripotent stem cell lines. Nature 450, E12–E14 (2007). https://doi.org/10.1038/nature06456
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DOI: https://doi.org/10.1038/nature06456
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