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Genotyping of Rhesus SCNT pluripotent stem cell lines

A Corrigendum to this article was published on 21 February 2008

Abstract

Arising from: J. A. Byrne et al. Nature 450, doi: 10.1038/nature06357 (2007)

Somatic cell nuclear transfer (SCNT) into enucleated oocytes has emerged as a technique that can be used to derive mouse embryonic stem cell lines with defined genotypes. In this issue Byrne et al.1 report the derivation of two SCNT Rhesus macaca male stem cell lines designated CRES-1 and CRES-2. Molecular studies detailed in their paper provides supporting evidence that the chromosome complement of CRES-1 and CRES-2 was genetically identical to the male cell donor nucleus and that the mitochondrial DNA originated from different recipient oocytes. In this validation paper, we independently confirm that both stem cell lines were indeed derived by SCNT.

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Figure 1: Analysis of nuclear and mitochondrial DNA origin in CRES-1 and CRES-2 stem cell lines by STR genotyping and DNA sequencing.

References

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Cram, D., Song, B. & Trounson, A. Genotyping of Rhesus SCNT pluripotent stem cell lines. Nature 450, E12–E14 (2007). https://doi.org/10.1038/nature06456

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