Pollinator shifts drive increasingly long nectar spurs in columbine flowers

Abstract

Directional evolutionary trends have long garnered interest because they suggest that evolution can be predictable. However, the identification of the trends themselves and the underlying processes that may produce them have often been controversial1. In 1862, in explaining the exceptionally long nectar spur of Angraecum sesquipedale, Darwin proposed that a coevolutionary ‘race’ had driven the directional increase in length of a plant’s spur and its pollinator’s tongue2. Thus he predicted the existence of an exceptionally long-tongued moth. Though the discovery of Xanthopan morgani ssp. praedicta in 1903 with a tongue length of 22 cm validated Darwin’s prediction3, his ‘race’ model for the evolution of long-spurred flowers remains contentious4. Spurs may also evolve to exceptional lengths by way of pollinator shifts as plants adapt to a series of unrelated pollinators, each with a greater tongue length5. Here, using a species-level phylogeny of the columbine genus, Aquilegia, we show a significant evolutionary trend for increasing spur length during directional shifts to pollinators with longer tongues. In addition, we find evidence for ‘punctuated’ change in spur length during speciation events6, suggesting that Aquilegia nectar spurs rapidly evolve to fit adaptive peaks predefined by pollinator morphology. These findings show that evolution may proceed in predictable pathways without reversals and that change may be concentrated during speciation.

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Figure 1: Two contrasting hypotheses for the evolution of exceptionally long nectar spurs.
Figure 2: Quantification of pollination syndromes and the distribution of spur lengths in Aquilegia.
Figure 3: Phylogenetic analysis of pollination syndrome evolution in Aquilegia.
Figure 4: Independent-contrasts regression analysis of pollination syndrome and spur-length evolution.

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Acknowledgements

We thank D. Bush and B. Counterman for laboratory assistance; J. Yang, M. Fulton, S. Schaefer, Y. Kisel and J. Bean for greenhouse and field help; T. Oakley and B. O’Meara for technical assistance; C. Voelkel and C. Eckert for comments on earlier versions of this manuscript; and M. Hodges for help with the figures. Funding was provided by the Botanical Society of America, UCSB’s Olivia Long Converse Fellowship, the National Science Foundation, UC Davis’ Comparative Biology Postdoctoral Fellowship, and the Native Plant Societies of Wyoming, Colorado, and northern Nevada.

Author Contributions J.B.W. and S.A.H. designed the study and collected the samples; J.B.W. collected and analysed the phylogenetic and comparative data; J.B.W. and S.A.H. wrote the paper.

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Correspondence to Justen B. Whittall or Scott A. Hodges.

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Reprints and permissions information is available at www.nature.com/reprints. The authors declare no competing financial interests.

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This file contains Supplementary Methods, Supplementary Discussion, Supplementary Figure 1 with Legend, Supplementary Tables 1-5 and additional references. (PDF 481 kb)

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Whittall, J., Hodges, S. Pollinator shifts drive increasingly long nectar spurs in columbine flowers. Nature 447, 706–709 (2007). https://doi.org/10.1038/nature05857

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