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Structural Biology

Analysis of 'downhill' protein folding; Analysis of protein-folding cooperativity (Reply)


Ferguson et al.1 and Zhou and Bai2 criticize the quality of our nuclear magnetic resonance (NMR) data and atom-by-atom analysis of global 'downhill' folding3, also claiming that the data are compatible with two-state folding.

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Figure 1: Analysis of the physical relevance of global two-state fits.


  1. Ferguson, N., Sharpe, T. D., Johnson, C. M., Schartau, P. J. & Fersht, A. R. Nature 445, doi: 10.1038/nature05643 (2007).

  2. Zhou, Z. & Bai, Y. Nature 445, doi: 10.1038/nature05644 (2007).

  3. Sadqi, M., Fushman, D. & Muñoz, V. Nature 442, 317–321 (2006).

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  4. Ferguson, N. et al. J. Mol. Biol. 353, 427–446 (2005).

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  5. Naganathan, A. N., Perez-Jimenez, R., Sanchez-Ruiz, J. M. & Muñoz, V. Biochemistry 44, 7435–7449 (2005).

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Sadqi, M., Fushman, D. & Muñoz, V. Analysis of 'downhill' protein folding; Analysis of protein-folding cooperativity (Reply). Nature 445, E17–E18 (2007).

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