Angiotensin -converting enzyme 2 (ACE2) is a regulator of the renin angiotensin system involved in acute lung failure, cardiovascular functions and severe acute respiratory syndrome (SARS) infections in mammals1,2,3. A gene encoding a homologue to ACE2, termed collectrin (Tmem27), has been identified in immediate proximity to the ace2 locus4. The in vivo function of collectrin was unclear. Here we report that targeted disruption of collectrin in mice results in a severe defect in renal amino acid uptake owing to downregulation of apical amino acid transporters in the kidney. Collectrin associates with multiple apical transporters and defines a novel group of renal amino acid transporters. Expression of collectrin in Xenopus oocytes and Madin–Darby canine kidney (MDCK) cells enhances amino acid transport by the transporter B0AT1. These data identify collectrin as a key regulator of renal amino acid uptake.

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We thank M. J. Crackower, A. Leibbrandt, Y. Imai, K. Kuba and many others for their contributions. Supported by grants from The National Bank of Austria, The Austrian Ministry of Science and Education, IMBA, an EU Marie Curie Excellence grant, and EUGeneHeart to J.M.P., from the Swiss National Science Foundation to F.V. and EUGINDAT to F.V. and C.A.W.

Author Contributions U.D, R.S., C.R., C.B., G.S., A.R., S.A., J.A.P., D.S., S.M.R.C., V.M., T.R. and C.A.W. performed experiments. U.D., F.V., C.A.W. and J.M.P. planned experiments and wrote the manuscript.

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  1. IMBA, Institute for Molecular Biotechnology of the Austrian Academy of Sciences, Dr. Bohr-Gasse 3, A-1030, Vienna, Austria

    • Ursula Danilczyk
    • , Renu Sarao
    • , J. Andrew Pospisilik
    •  & Josef M. Penninger
  2. Institute of Physiology and Center for Integrative Human Physiology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland

    • Christine Remy
    • , Chahira Benabbas
    • , Gerti Stange
    • , Dustin Singer
    • , Simone M. R. Camargo
    • , Victoria Makrides
    • , Tamara Ramadan
    • , Francois Verrey
    •  & Carsten A. Wagner
  3. Department of Chemical Ecology and Ecosystem Research, Center of Ecology, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria

    • Andreas Richter
  4. The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada

    • Sudha Arya


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Correspondence to Carsten A. Wagner or Josef M. Penninger.

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