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An RNA gene expressed during cortical development evolved rapidly in humans

Abstract

The developmental and evolutionary mechanisms behind the emergence of human-specific brain features remain largely unknown. However, the recent ability to compare our genome to that of our closest relative, the chimpanzee, provides new avenues to link genetic and phenotypic changes in the evolution of the human brain. We devised a ranking of regions in the human genome that show significant evolutionary acceleration. Here we report that the most dramatic of these ‘human accelerated regions’, HAR1, is part of a novel RNA gene (HAR1F) that is expressed specifically in Cajal–Retzius neurons in the developing human neocortex from 7 to 19 gestational weeks, a crucial period for cortical neuron specification and migration. HAR1F is co-expressed with reelin, a product of Cajal–Retzius neurons that is of fundamental importance in specifying the six-layer structure of the human cortex. HAR1 and the other human accelerated regions provide new candidates in the search for uniquely human biology.

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Figure 1: HAR1-associated transcripts in genomic context.
Figure 2: Predicted RNA secondary structure for HAR1F.
Figure 3: Expression of HAR1F and HAR1R in the developing neocortex.
Figure 4: Expression of HAR1F in other parts of the developing brain.

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Acknowledgements

We thank G. Bejerano, C. Lowe, J. Kent, H. Noller, D. Feldheim, A. Love (UCSC), V. Albert and J.-C. Noel (Erasme Hospital), J.-P. Brion (ULB), A. Goffinet (UCL Louvain), the UCSC Genome Browser Group, Webb Miller (Penn. State), the Macaque Genome Sequencing Consortium, and the Broad Institute Genome Sequencing Platform and Whole-Genome Assembly Team. This work was funded by the Howard Hughes Medical Institute (D.H., S.R.S. and B.K.), the US NHGRI (D.H., A.D.K., S.K. and C.O.), the US National Cancer Institute (J.S.P.), the US NIGMS (K.S.P. and M.A.), the University of California Biotechnology Research and Education Program (A.S.), the Danish Research Council (J.S.P.), the Belgian FNRS, the Belgian FRSM, the Belgian Queen Elizabeth Medical Foundation (FMRE), the Interuniversity Attraction Poles Programme, Belgian State and Federal Office for Scientific, Technical and Cultural Affairs (P.V.), and the Fondation Erasme (M.-A.L. and N.L.). P.V. and M.-A.L. are Research Associate and Research Fellow of the FNRS, respectively. Author Contributions Computational methods developed and applied by K.S.P., J.S.P., A.S. and A.D.K. Experimental analysis by S.R.S., P.V., N.L., M.-A.L., S.C., S.K., B.K., C.O., C.D., H.I. and M.A. Manuscript primarily written by K.S.P., S.R.S., P.V. and D.H.

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Correspondence to David Haussler.

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The sequences of the HAR1 transcripts reported in this study have been submitted to GenBank (accession numbers DQ860409–DQ860415). Reprints and permissions information is available at npg.nature.com/reprintsandpermissions. The authors declare no competing financial interests.

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Pollard, K., Salama, S., Lambert, N. et al. An RNA gene expressed during cortical development evolved rapidly in humans. Nature 443, 167–172 (2006). https://doi.org/10.1038/nature05113

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