Letter | Published:

Dynamics of heat shock factor association with native gene loci in living cells

Abstract

Direct observation of transcription factor action in the living cell nucleus can provide important insights into gene regulatory mechanisms1,2. Live-cell imaging techniques have enabled the visualization of a variety of intranuclear activities, from chromosome dynamics3 to gene expression4. However, progress in studying transcription regulation of specific native genes has been limited, primarily as a result of difficulties in resolving individual gene loci and in detecting the small number of protein molecules functioning within active transcription units. Here we report that multiphoton microscopy imaging5 of polytene nuclei in living Drosophila salivary glands allows real-time analysis of transcription factor recruitment and exchange on specific native genes. After heat shock, we have visualized the recruitment of RNA polymerase II (Pol II) to native hsp70 gene loci 87A and 87C in real time. We show that heat shock factor (HSF), the transcription activator of hsp70, is localized to the nucleus before heat shock and translocates from nucleoplasm to chromosomal loci after heat shock. Assays based on fluorescence recovery after photobleaching6 show a rapid exchange of HSF at chromosomal loci under non-heat-shock conditions but a very slow exchange after heat shock. However, this is not a consequence of a change of HSF diffusibility, as shown here directly by fluorescence correlation spectroscopy7. Our results provide strong evidence that activated HSF is stably bound to DNA in vivo and that turnover or disassembly of transcription activator is not required for rounds of hsp70 transcription. This and previous studies8,9 indicate that transcription activators display diverse dynamic behaviours in their associations with targeted loci in living cells. Our method can be applied to study the dynamics of many factors involved in transcription and RNA processing, and in their regulation at native heat shock genes in vivo.

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Acknowledgements

We thank J. K. Werner for immunofluorescence labeling of polytene chromosomes; K. L. Zobeck for initial western blot experiments; R. M. Williams and W. R. Zipfel for technical assistance with MPM imaging; T. Kodadek for exchanging manuscripts before submission; and W. L. Kraus for critical reading of the manuscript. This work was performed partly in the Developmental Resources for Biophysical Imaging Opto-Electronics and was supported by an NSF grant to W.W.W. and J.T.L., an NIH grant to J.T.L., and an NIH–NIBIB grant to W.W.W.

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Correspondence to Watt W. Webb or John T. Lis.

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Reprints and permissions information is available at www.nature.com/reprints. The authors declare no competing financial interests.

Supplementary information

Supplementary Figures

This file contains Supplementary Figures 1-8 with their legends. (PDF 2248 kb)

Supplementary Notes

This file contains Supplementary Methods, Supplementary Discussions, Supplementary Movie Legend, and Supplementary Figure S9 with legend. (PDF 638 kb)

Supplementary Movie 1

This movie shows the recruitment of HSF–EGFP to chromosomal loci as a response to heat shock in living polytene cells. (MOV 312 kb)

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Figure 1: Multiphoton imaging of Drosophila polytene nuclei in living cells.
Figure 2: Expression and localization of HSF–EGFP, and translocation of HSF–EGFP from nucleoplasm to chromosomal loci upon HS.
Figure 3: Distinct exchange kinetics of HSF at specific chromosomal loci during NHS and HS conditions.

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