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Nuclear reprogramming and pluripotency

Naturevolume 441pages10611067 (2006) | Download Citation

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Abstract

The cloning of mammals from differentiated donor cells has refuted the old dogma that development is an irreversible process. It has demonstrated that the oocyte can reprogramme an adult nucleus into an embryonic state that can direct development of a new organism. The prospect of deriving patient-specific embryonic stem cells by nuclear transfer underscores the potential use of this technology in regenerative medicine. The future challenge will be to study alternatives to nuclear transfer in order to recapitulate reprogramming in a Petri dish without the use of oocytes.

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Acknowledgements

We would like to thank B. Hogan, L. Looijenga, A. Wutz, N. Geijsen, M. W. Lensch and members of the Jaenisch laboratory for discussion and critical reading of the manuscript. Support to K.H. came from a Genzyme postdoctoral fellowship. R.J. is supported by grants from the National Institutes of Health.

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    • Konrad Hochedlinger

    Present address: Center for Regenerative Medicine and Cancer Center, Massachusetts General Hospital, Harvard Medical School and Harvard Stem Cell Institute, 185 Cambridge Street, Boston, Massachusetts, 02114, USA

Affiliations

  1. Department of Biology, Massachusetts Institute of Technology, and Whitehead Institute, 9 Cambridge Center, Cambridge, 02142, Massachusetts, USA

    • Konrad Hochedlinger
    •  & Rudolf Jaenisch

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Correspondence to Rudolf Jaenisch.

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