Figure 2: Circulating cells do not give rise to mature oocytes in injured parabionts. | Nature

Figure 2: Circulating cells do not give rise to mature oocytes in injured parabionts.

From: Ovulated oocytes in adult mice derive from non-circulating germ cells

Figure 2

a, Experimental design. GFP-transgenic and non-transgenic C57BL/Ka mice were surgically joined by parabiosis 1 day after treatment of the non-transgenic partner only with the ovotoxic agents cyclophosphamide (Cy) and busulfan (Bu). After 10 days or 2 months of shared circulation, pairs were treated with PMS and HCG, and analysed for the presence of cross-engrafting oocytes and/or germ cells. b, Representative brightfield (left) and epifluorescence (right) images of oocytes collected after 2 weeks of parabiosis from the GFP-transgenic or non-transgenic partners of untreated pair 5, in which the non-transgenic partner was left untreated, or Cy/Bu-treated pair 8, in which the non-transgenic partner was pre-treated with Cy/Bu (Supplementary Table 2). Total numbers of GFP+ oocytes per total oocytes examined are summarized on the epifluorescence panels for each group (n = 4 parabiotic pairs). c, Average number of oocytes (± s.d.) collected from parabiotic mice 2 weeks after injury and parabiosis. Black bars represent data from pairs in which neither partner received Cy/Bu treatment; grey bars represent data from pairs in which the non-transgenic partner only received Cy/Bu treatment. All oocytes from GFP-transgenic parabionts expressed GFP, whereas no oocytes from their non-transgenic partners expressed GFP (see Supplementary Table 2). d, Representative brightfield (left) and epifluorescence (right) images of oocytes collected after 2 months of parabiosis from the GFP-transgenic or non-transgenic partners of untreated pair 1, in which the non-transgenic partner was left untreated, or pooled from Cy/Bu-treated pairs 1 and 2, in which the non-transgenic partner was pre-treated with Cy/Bu (Supplementary Table 2). Total numbers of GFP+ oocytes per total oocytes examined are summarized on the epifluorescence panels for each group (n = 2 parabiotic pairs). e, Average number of oocytes (± s.d.) collected from parabiotic mice 2 months after injury and parabiosis. Black bars represent data from pairs in which neither partner received Cy/Bu treatment; grey bars represent data from pairs in which the non-transgenic partner only received Cy/Bu treatment. All oocytes from GFP-transgenic parabionts expressed GFP, whereas no oocytes from their non-transgenic partners expressed GFP (see Supplementary Table 2).

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