Nature 428, 717–723 (2004)
In this Article, we showed that Pitx1 maps to the major locus controlling pelvic reduction in sticklebacks and shows altered expression, but not altered amino-acid sequence, in a pelvic-reduced population. Owing to a technical error, Figs 3 and 4 of the Article incorrectly show results from a Pitx1 sense probe, rather than from an antisense probe. Similar staining is not observed for sense probes from other genes, raising the possibility that sticklebacks may express some endogenous antisense transcripts from the Pitx1 region.
A new antisense probe (see Fig. 1, below) shows strong expression in the mouth, lower jaw and developing pelvis of marine larvae. Pitx1 expression appears normal in the mouth and lower jaw of the Paxton benthic population, but is completely missing in the pelvic region. No expression was detected in neuromasts, thymus, olfactory pits or tail in either population, unlike the pattern seen with the original probe. Despite these differences, our major conclusion, that pelvic reduction results from cis-acting regulatory changes in the Pitx1 locus, remains unchanged. This is because the endogenous sense transcript loses its expression along with the presumptive antisense transcript in the pelvic region of the larval progeny of fish missing the pelvis.
The online version of the original article can be found at 10.1038/nature02415
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Shapiro, M., Marks, M., Peichel, C. et al. Correction: Corrigendum: Genetic and developmental basis of evolutionary pelvic reduction in threespine sticklebacks. Nature 439, 1014 (2006) doi:10.1038/nature04500
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