DNA primase acts as a molecular brake in DNA replication

Abstract

A hallmark feature of DNA replication is the coordination between the continuous polymerization of nucleotides on the leading strand and the discontinuous synthesis of DNA on the lagging strand1. This synchronization requires a precisely timed series of enzymatic steps that control the synthesis of an RNA primer, the recycling of the lagging-strand DNA polymerase, and the production of an Okazaki fragment. Primases synthesize RNA primers at a rate that is orders of magnitude lower2,3,4 than the rate of DNA synthesis by the DNA polymerases at the fork. Furthermore, the recycling of the lagging-strand DNA polymerase from a finished Okazaki fragment to a new primer is inherently slower than the rate of nucleotide polymerization5. Different models have been put forward to explain how these slow enzymatic steps can take place at the lagging strand without losing coordination with the continuous and fast leading-strand synthesis6,7,8. Nonetheless, a clear picture remains elusive. Here we use single-molecule techniques to study the kinetics of a multiprotein replication complex from bacteriophage T7 and to characterize the effect of primase activity on fork progression. We observe the synthesis of primers on the lagging strand to cause transient pausing of the highly processive leading-strand synthesis. In the presence of both leading- and lagging-strand synthesis, we observe the formation and release of a replication loop on the lagging strand. Before loop formation, the primase acts as a molecular brake and transiently halts progression of the replication fork. This observation suggests a mechanism that prevents leading-strand synthesis from outpacing lagging-strand synthesis during the slow enzymatic steps on the lagging strand.

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Figure 1: The T7 replication fork.
Figure 2: Experimental design.
Figure 3: Single-molecule observation of T7 leading-strand synthesis.
Figure 4: Lagging-strand synthesis and loop formation.

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Acknowledgements

We wish to thank T. Ellenberger, D. Crampton and P. Blainey for discussions and comments, and S. Buratowski for critically reading the manuscript. We are grateful to S.-J. Lee for providing the purified ZBD-less gp4 variant. We thank S. Moskowitz for preparation of the figures. This work was supported by grants from the NIH to C.C.R. and X.S.X.

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Correspondence to Antoine M. van Oijen.

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This file contains Supplementary Methods, Supplementary Figures S1−S9, and additional references. (PDF 487 kb)

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Lee, J., Hite, R., Hamdan, S. et al. DNA primase acts as a molecular brake in DNA replication. Nature 439, 621–624 (2006). https://doi.org/10.1038/nature04317

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