Direct dating of Early Upper Palaeolithic human remains from Mladeč


The human fossil assemblage from the Mladeč Caves in Moravia (Czech Republic)1 has been considered to derive from a middle or later phase of the Central European Aurignacian period on the basis of archaeological remains (a few stone artefacts and organic items such as bone points, awls, perforated teeth)2, despite questions3 of association between the human fossils and the archaeological materials and concerning the chronological implications of the limited archaeological remains4. The morphological variability in the human assemblage, the presence of apparently archaic features in some specimens, and the assumed early date of the remains have made this fossil assemblage pivotal in assessments of modern human emergence within Europe5,6,7. We present here the first successful direct accelerator mass spectrometry radiocarbon dating of five representative human fossils from the site. We selected sample materials from teeth and from one bone for 14C dating. The four tooth samples yielded uncalibrated ages of 31,000 14C years before present, and the bone sample (an ulna) provided an uncertain more-recent age. These data are sufficient to confirm that the Mladeč human assemblage is the oldest cranial, dental and postcranial assemblage of early modern humans in Europe and is therefore central to discussions of modern human emergence in the northwestern Old World and the fate of the Neanderthals.

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Figure 1: Dated human fossil specimens from Mladeč.
Figure 2: Documentation of the dated specimens showing the sampled parts in red.


  1. 1

    Szombathy, J. Die diluvialen Menschenreste aus der Fürst-Johanns-Höhle bei Lautsch in Mähren. Die Eiszeit 2, 1–34, 73–95 (1925)

    Google Scholar 

  2. 2

    Oliva, M. UISPP XIIe Congres Vol. 2, 207–216 (Institut d'Archéologie, Bratislava, 1993)

    Google Scholar 

  3. 3

    Conard, N. J., Grootes, P. M. & Smith, F. H. Unexpectedly recent dates for human remains from Vogelherd. Nature 430, 198–200 (2004)

    ADS  CAS  Article  Google Scholar 

  4. 4

    Svoboda, J. The depositional context of the Early Upper Paleolithic human fossils from the Koněprusy (Zlatý kůň) and Mladeč Caves, Czech Republic. J. Hum. Evol. 38, 523–536 (2000)

    CAS  Article  Google Scholar 

  5. 5

    Frayer, D. W. Cranial variation at Mladeč and the relationship between Mousterian and Upper Paleolithic hominids. Anthropos 23, 243–256 (1986)

    Google Scholar 

  6. 6

    Wolpoff, M. H., Hawks, J., Fayer, D. W. & Hunley, K. Modern human ancestry at the peripheries: A test of the replacement theory. Science 291, 293–297 (2001)

    ADS  CAS  Article  Google Scholar 

  7. 7

    Bräuer, G., Collard, M. & Stringer, C. On the reliability of recent tests of the Out of Africa hypothesis for modern human origins. Anat. Rec. 279A, 701–707 (2004)

    Article  Google Scholar 

  8. 8

    Mellars, P. Neanderthals and the modern human colonization of Europe. Nature 432, 461–465 (2004)

    ADS  CAS  Article  Google Scholar 

  9. 9

    Svoboda, J. A., van der Plicht, J. & Kuzelka, V. Upper Palaeolithic and Mesolithic human fossils from Moravia and Bohemia (Czech Republic): some new 14C dates. Antiquity 76, 957–962 (2002)

    Article  Google Scholar 

  10. 10

    Smith, F. H., Trinkaus, E., Pettitt, P. B., Karavanić, I. & Paunović, M. Direct radiocarbon dates for Vindija G1 and Velika Pećina Late Pleistocene hominid remains. Proc. Natl Acad. Sci. USA 96, 12281–12286 (1999)

    ADS  CAS  Article  Google Scholar 

  11. 11

    Terberger, T., Street, M. & Bräuer, G. Der menschliche Schädelrest aus der Elbe bei Hahnöfersand und seine Bedeutung für die Steinzeit Norddeutschlands. Archäol. Korrespond. 31, 521–526 (2001)

    Google Scholar 

  12. 12

    Henry-Gambier, D. Les fossils de Cro-Magnon (Les Eyzies-de-Tayac, Dordogne): nouvelles données sur leur position chronologique et leur attribution culturelle. Bull. Mem. Soc. Anthropol. Paris 14, 89–112 (2002)

    Google Scholar 

  13. 13

    Orschiedt, J. Datation d'un vestige humain provenant de La Rochette (Saint Léon-sur-Vézère, Dordogne) par la méthode du carbone 14 en spectrométrie de masse. Paléo 14, 239–240 (2002)

    Google Scholar 

  14. 14

    Trinkaus, E. et al. An early modern human from the Peştera cu Oase, Romania. Proc. Natl Acad. Sci. USA 100, 11231–11236 (2003)

    ADS  CAS  Article  Google Scholar 

  15. 15

    Stringer, C. B. In Hominid Remains: An Update. British Isles and Eastern Germany (ed. Orban, R.) 1–40 (Université Libre de Bruxelles, Brussels, 1990)

    Google Scholar 

  16. 16

    Păunescu, A. Paleoliticul şi Mezoliticul din Spaţiul Transilvan 231 (Editura AGIR, Bucharest, 2001)

    Google Scholar 

  17. 17

    Hublin, J. J., Spoor, F., Braun, M., Zonneveld, F. & Condemi, S. A late Neanderthal associated with Upper Palaeolithic artefacts. Nature 381, 224–226 (1996)

    ADS  CAS  Article  Google Scholar 

  18. 18

    Serre, D. et al. No evidence of Neanderthal mtDNA contribution to early modern humans. PLoS Biol. 2, e57 (2004)

    Article  Google Scholar 

  19. 19

    Schmitz, R. et al. The Neanderthal type site revisited: Interdisciplinary investigations of skeletal remains from the Neander Valley, Germany. Proc. Natl Acad. Sci. USA 99, 13342–13347 (2002)

    ADS  CAS  Article  Google Scholar 

  20. 20

    Wild, E. M. et al. First 14C results from archaeological and forensic studies at the Vienna Environmental Research Accelerator. Radiocarbon 40, 273–281 (1998)

    CAS  Article  Google Scholar 

  21. 21

    Steier, P. et al. Pushing the precision limit of 14C AMS. Radiocarbon 46, 5–16 (2004)

    CAS  Article  Google Scholar 

  22. 22

    Bard, E., Rostek, F. & Ménot-Combes, G. A better radiocarbon clock. Science 303, 178–179 (2004)

    CAS  Article  Google Scholar 

  23. 23

    van Andel, T. H., Davies, W., Weninger, B. & Jöris, B. In Neanderthals and Modern Humans in the European Landscape During the Last Glaciation: Archaeological Results of the Stage 3 Project (eds van Andel, T. H. & Davies, W.) 21–31 (Oxbow, Oxford, 2004)

    Google Scholar 

  24. 24

    van der Plicht, J. et al. NOTCAL04-Comparison/calibration 14C records 26–50 cal kyr BP. Radiocarbon 46, 1–14 (2004)

    Article  Google Scholar 

  25. 25

    Hedges, R. E. M. & van Klinken, G. J. A review of current approaches in the pretreatment of bone for radiocarbon dating by AMS. Radiocarbon 34, 279–291 (1992)

    CAS  Article  Google Scholar 

  26. 26

    Petchey, F. in Chemical Pretreatment Methods (ed. Higham, T.)

  27. 27

    DeNiro, M. J. Postmortem preservation and alteration of in vivo bone collagen isotope ratios in relation to palaeodietary reconstruction. Nature 317, 806–809 (1985)

    ADS  CAS  Article  Google Scholar 

  28. 28

    Ambrose, S. H. Preparation and characterization of bone and tooth for isotopic analysis. J. Archaeol. Sci. 17, 431–451 (1990)

    Article  Google Scholar 

  29. 29

    Schoeninger, M. J., Moore, K. M., Murray, M. L. & Kingston, J. D. Detection of bone preservation in archaeological and fossil samples. Appl. Geochem. 4, 281–292 (1989)

    CAS  Article  Google Scholar 

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We thank D. Frayer and G. Kurat for comments on an earlier version of this paper. We also thank H. Prossinger and other colleagues for discussions. Furthermore we thank W. Reichmann for the photographic documentation, R. Mühl and A. Walch for technical support, and S. Lehr for his help in sample preparation.

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Correspondence to Eva M. Wild or Maria Teschler-Nicola.

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Reprints and permissions information is available at The authors declare no competing financial interests.

Supplementary information

Supplementary Table S1

Radiocarbon ages determined for the animal remains from the Mladec site. (DOC 34 kb)

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Wild, E., Teschler-Nicola, M., Kutschera, W. et al. Direct dating of Early Upper Palaeolithic human remains from Mladeč. Nature 435, 332–335 (2005).

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