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X-inactivation profile reveals extensive variability in X-linked gene expression in females


In female mammals, most genes on one X chromosome are silenced as a result of X-chromosome inactivation1,2. However, some genes escape X-inactivation and are expressed from both the active and inactive X chromosome. Such genes are potential contributors to sexually dimorphic traits, to phenotypic variability among females heterozygous for X-linked conditions, and to clinical abnormalities in patients with abnormal X chromosomes3. Here, we present a comprehensive X-inactivation profile of the human X chromosome, representing an estimated 95% of assayable genes in fibroblast-based test systems4,5. In total, about 15% of X-linked genes escape inactivation to some degree, and the proportion of genes escaping inactivation differs dramatically between different regions of the X chromosome, reflecting the evolutionary history of the sex chromosomes. An additional 10% of X-linked genes show variable patterns of inactivation and are expressed to different extents from some inactive X chromosomes. This suggests a remarkable and previously unsuspected degree of expression heterogeneity among females.

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Figure 1: Q-SNaPshot assay of allelic expression for three X-linked genes.
Figure 2: X-inactivation as a measure of allelic expression in non-randomly inactivated primary fibroblasts.
Figure 3: X-inactivation profile of the human X chromosome.
Figure 4: Xi expression data in primary fibroblasts and Xi hybrids correlate with location on X.

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  1. Lyon, M. F. Gene action in the X-chromosome of the mouse (Mus musculus L.). Nature 190, 372–373 (1961)

    Article  ADS  CAS  Google Scholar 

  2. Plath, K., Mlynarczyk-Evans, S., Nusinow, D. A. & Panning, B. Xist RNA and the mechanism of X chromosome inactivation. Annu. Rev. Genet. 36, 233–278 (2002)

    Article  CAS  Google Scholar 

  3. Willard, H. F. in The Metabolic and Molecular Bases of Inherited Disease 8th edn (eds Scriver, C. R., Beaudet, A. L., Sly, W. S., Valle, D., Childs, B. & Vogelstein, B.) 1191–1221 (McGraw-Hill, New York, 2000)

    Google Scholar 

  4. Carrel, L., Cottle, A. A., Goglin, K. C. & Willard, H. F. A first-generation X-inactivation profile of the human X chromosome. Proc. Natl Acad. Sci. USA 96, 14440–14444 (1999)

    Article  ADS  CAS  Google Scholar 

  5. Carrel, L. & Willard, H. F. Heterogeneous gene expression from the inactive X chromosome: an X-linked gene that escapes X inactivation in some human cell lines but is inactivated in others. Proc. Natl Acad. Sci. USA 96, 7364–7369 (1999)

    Article  ADS  CAS  Google Scholar 

  6. Brown, C. J. et al. A gene from the region of the human X inactivation centre is expressed exclusively from the inactive X chromosome. Nature 349, 38–44 (1991)

    Article  ADS  CAS  Google Scholar 

  7. Penny, G. D., Kay, G. F., Sheardown, S. A., Rastan, S. & Brockdorff, N. Requirement for Xist in X chromosome inactivation. Nature 379, 131–137 (1996)

    Article  ADS  CAS  Google Scholar 

  8. Rougeulle, C. et al. Differential histone H3 Lys-9 and Lys-27 methylation profiles on the X chromosome. Mol. Cell. Biol. 24, 5475–5484 (2004)

    Article  CAS  Google Scholar 

  9. Chadwick, B. P. & Willard, H. F. Barring gene expression after XIST: maintaining facultative heterochromatin on the inactive X. Semin. Cell Dev. Biol. 14, 359–367 (2003)

    Article  CAS  Google Scholar 

  10. Chadwick, B. P. & Willard, H. F. Multiple spatially distinct types of facultative heterochromatin on the human inactive X chromosome. Proc. Natl Acad. Sci. USA 101, 17450–17455 (2004)

    Article  ADS  CAS  Google Scholar 

  11. Lyon, M. F. Sex chromatin and gene action in the mammalian X-chromosome. Am. J. Hum. Genet. 14, 135–148 (1962)

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Brown, C. J. & Greally, J. M. A stain upon the silence: genes escaping X inactivation. Trends Genet. 19, 432–438 (2003)

    Article  CAS  Google Scholar 

  13. Ross, M. T. et al. The DNA sequence of the human X chromosome. Nature doi:10.1038/nature03440 (this issue).

  14. Miller, A. P. & Willard, H. F. Chromosomal basis of X chromosome inactivation: identification of a multigene domain in Xp11.21-p11.22 that escapes X inactivation. Proc. Natl Acad. Sci. USA 95, 8709–8714 (1998)

    Article  ADS  CAS  Google Scholar 

  15. Huynh, K. D. & Lee, J. T. Inheritance of a pre-inactivated paternal X chromosome in early mouse embryos. Nature 426, 857–862 (2003)

    Article  ADS  CAS  Google Scholar 

  16. Ohno, S. Sex Chromosomes and Sex-linked Genes (Springer, Berlin, 1967)

    Book  Google Scholar 

  17. Skaletsky, H. et al. The male-specific region of the human Y chromosome is a mosaic of discrete sequence classes. Nature 423, 825–837 (2003)

    Article  ADS  CAS  Google Scholar 

  18. Graves, J. A. Mammals that break the rules: genetics of marsupials and monotremes. Annu. Rev. Genet. 30, 233–260 (1996)

    Article  CAS  Google Scholar 

  19. Lahn, B. T. & Page, D. C. Four evolutionary strata on the human X chromosome. Science 286, 964–967 (1999)

    Article  CAS  Google Scholar 

  20. Jegalian, K. & Page, D. C. A proposed path by which genes common to mammalian X and Y chromosomes evolve to become X inactivated. Nature 394, 776–780 (1998)

    Article  ADS  CAS  Google Scholar 

  21. Kohn, M., Kehrer-Sawatzki, H., Vogel, W., Graves, J. A. & Hameister, H. Wide genome comparisons reveal the origins of the human X chromosome. Trends Genet. 20, 598–603 (2004)

    Article  CAS  Google Scholar 

  22. Gartler, S. M. & Riggs, A. D. Mammalian X-chromosome inactivation. Annu. Rev. Genet. 17, 155–190 (1983)

    Article  CAS  Google Scholar 

  23. Lyon, M. F. X-chromosome inactivation: a repeat hypothesis. Cytogenet. Cell Genet. 80, 133–137 (1998)

    Article  CAS  Google Scholar 

  24. Bailey, J. A., Carrel, L., Chakravarti, A. & Eichler, E. E. Molecular evidence for a relationship between LINE-1 elements and X chromosome inactivation: the Lyon repeat hypothesis. Proc. Natl Acad. Sci. USA 97, 6634–6639 (2000)

    Article  ADS  CAS  Google Scholar 

  25. Mohandas, T., Sparkes, R. S. & Shapiro, L. J. Reactivation of an inactive human X chromosome: evidence for X inactivation by DNA methylation. Science 211, 393–396 (1981)

    Article  ADS  CAS  Google Scholar 

  26. Ke, X. & Collins, A. CpG islands in human X-inactivation. Ann. Hum. Genet. 67, 242–249 (2003)

    Article  CAS  Google Scholar 

  27. Committee on Understanding the Biology of Sex and Gender Differences. In Exploring the Biological Contributions to Human Health–Does Sex Matter? (eds Wizemann, T. M. & Pardue, M.) (National Acadamies Press, Washington, DC, 2001)

    Google Scholar 

  28. Iyer, V. R. et al. The transcriptional program in the response of human fibroblasts to serum. Science 283, 83–87 (1999)

    Article  ADS  CAS  Google Scholar 

  29. Esposito, T. et al. Escape from X inactivation of two new genes associated with DXS6974E and DXS7020E. Genomics 43, 183–190 (1997)

    Article  CAS  Google Scholar 

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We thank M. Ross for sharing information before publication and gratefully acknowledge technical assistance from G. Nickel, K. Trevarthen, J. Dunn, A. Cottle and M. Moon. This work was supported in part by a National Institutes of Health research grant to H.F.W. and L.C.

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Correspondence to Laura Carrel.

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Supplementary information

Supplementary Figure S1

Y homology influences Xi expression. (DOC 23 kb)

Supplementary Figure S2

Xi expression data correlate with repetitive element composition. (DOC 23 kb)

Supplementary Figure S3

Gene organization and sequence composition of 500 kb regions on the X; repetitive elements cannot fully explain Xi expression patterns. (PDF 107 kb)

Supplementary Figure S4

Distribution of transcripts with CpG islands. (DOC 23 kb)

Supplementary Figure S5

Levels of relative Xa and Xi expression. (DOC 63 kb)

Supplementary Figure S6

RT-PCR assays of gene expression from the Xi chromosome. (DOC 357 kb)

Supplementary Table S1

Genes assayed by quantitative analysis of expressed polymorphisms in panel of non-randomly inactivated primary fibroblasts. (XLS 40 kb)

Supplementary Table S2

X-linked genes analyzed in this study. (XLS 16 kb)

Supplementary Table S3

Genes analysed in Xi hybrids. (XLS 476 kb)

Supplementary Table S4

Comparison of X inactivation patterns as measured in primary fibroblasts and in Xi hybrids. (XLS 16 kb)

Supplementary Note S1

Notes as to why some reported genes were not fully analysed. (DOC 23 kb)

Supplementary Note S2

Although the RT-PCR assay utilized is not strictly quantitative, substantial effort was made to ensure that Xi hybrids scored positive did have significant levels of expression. (DOC 20 kb)

Supplementary Note S3

The S3/S4 boundary has been assigned to a 2.5 Mb region within Xp22.2-Xp22.31. (DOC 19 kb)

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Carrel, L., Willard, H. X-inactivation profile reveals extensive variability in X-linked gene expression in females. Nature 434, 400–404 (2005).

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