Letter | Published:

Spermatid differentiation requires the assembly of a cell polarity complex downstream of junctional adhesion molecule-C

Nature volume 431, pages 320324 (16 September 2004) | Download Citation

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Abstract

During spermatogenesis in the mammalian testis, stem cells (spermatogonia) differentiate into spermatocytes, which subsequently undergo two consecutive meiotic divisions to give rise to haploid spermatids. These cells are initially round but progressively elongate, condense their nuclei, acquire flagellar and acrosomal structures, and shed a significant amount of their cytoplasm to form spermatozoa (the sperm cells) in a developmental cascade termed spermiogenesis1,2. Defects in these processes will lead to a lack of mature sperm cells (azoospermia), which is a major cause of male infertility in the human population3. Here we report that a cell-surface protein of the immunoglobulin superfamily, junctional adhesion molecule-C (JAM-C), is critically required for the differentiation of round spermatids into spermatozoa in mice. We found that Jam-C is essential for the polarization of round spermatids, a function that we attribute to its role in the assembly of a cell polarity complex.

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Acknowledgements

We thank B. Margolis, T. Pawson, E. Peles, A. Suzuki, A. Le Bivic, C. Nobes and P. Parker for reagents and N. Hogg for comments on the manuscript. This work was funded by Cancer Research UK, the EMBO Young Investigator Program (R.H.A.), the Swiss National Science Foundation (M.A.L. and B.A.I.) and Oncosuisse (B.A.I).

Author information

Affiliations

  1. Vascular Development Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, UK

    • Georgia Gliki
    •  & Ralf H. Adams
  2. Institute of Cell Biology, ZMBE, University Hospital Muenster, D-48149 Muenster, Germany

    • Klaus Ebnet
  3. University of Geneva, CMU, Department of Pathology, CH-1211 Geneva, Switzerland

    • Michel Aurrand-Lions
    •  & Beat A. Imhof

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Competing interests

The authors declare that they have no competing financial interests.

Corresponding author

Correspondence to Ralf H. Adams.

Supplementary information

PDF files

  1. 1.

    Supplementary Figure 1

    JAM-C gene targeting strategy and verification of the null allele.

  2. 2.

    Supplementary Figure 2

    Histology showing the lack of mature spermatozoa in the epididymis of JAM-C-deficient mice.

  3. 3.

    Supplementary Figure 3

    Data confirming the integrity of mutant Sertoli-Sertoli tight junctions.

  4. 4.

    Supplementary Figure 4

    Additional immunofluorescence data for cell polarity proteins.

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DOI

https://doi.org/10.1038/nature02877

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