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The principle of temperature-dependent gating in cold- and heat-sensitive TRP channels

Nature volume 430, pages 748754 (12 August 2004) | Download Citation

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Abstract

The mammalian sensory system is capable of discriminating thermal stimuli ranging from noxious cold to noxious heat. Principal temperature sensors belong to the TRP cation channel family, but the mechanisms underlying the marked temperature sensitivity of opening and closing (‘gating’) of these channels are unknown. Here we show that temperature sensing is tightly linked to voltage-dependent gating in the cold-sensitive channel TRPM8 and the heat-sensitive channel TRPV1. Both channels are activated upon depolarization, and changes in temperature result in graded shifts of their voltage-dependent activation curves. The chemical agonists menthol (TRPM8) and capsaicin (TRPV1) function as gating modifiers, shifting activation curves towards physiological membrane potentials. Kinetic analysis of gating at different temperatures indicates that temperature sensitivity in TRPM8 and TRPV1 arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. Our results suggest a simple unifying principle that explains both cold and heat sensitivity in TRP channels.

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Acknowledgements

We thank K. Talavera, G. Owsianik, J. Vriens, F. Mahieu and J. Prenen for helpful suggestions and criticisms, and C. Benham (GlaxoSmithKline) for providing us with the human TRPV1 clone. This work was supported by the Belgian Federal Government, the Flemish Government and the Onderzoeksraad KU Leuven. T.V. is a postdoctoral Fellow of the Fund for Scientific Research, Flanders (Belgium) (FWO-Vlaanderen).

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Affiliations

  1. Laboratory of Physiology, Campus Gasthuisberg, KU Leuven, B-3000 Leuven, Belgium

    • Thomas Voets
    • , Guy Droogmans
    • , Annelies Janssens
    •  & Bernd Nilius
  2. Experimentelle und Klinische Pharmakologie und Toxikologie, Medizinische Fakultät, Universität des Saarlandes, D-66421 Homburg, Germany

    • Ulrich Wissenbach
    •  & Veit Flockerzi

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Competing interests

The authors declare that they have no competing financial interests.

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Correspondence to Thomas Voets.

Supplementary information

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    Supplementary Figure

    This figure shows examples of the detailed kinetic analysis of current relaxations for TRPM8 and TRPV1 at different temperatures and voltages.

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https://doi.org/10.1038/nature02732

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