Figure 2: Characterization of xylogen. | Nature

Figure 2: Characterization of xylogen.

From: A proteoglycan mediates inductive interaction during plant vascular development

Figure 2

a, Deduced amino acid sequences. Shown are the signal sequences (blue), AGP-like motifs (red), nsLTP-like cysteines (purple), N-glycosylation sites (green), identical residues (asterisks), cleavage sites followed by the GPI anchor (arrow), sequenced N-terminal amino acids (underlined) and hydroxylated prolines (circles). b, Domain structures of xylogen. PA, (Pro-Ala)-rich domain; TM, transmembrane domain. c, Transgenic BY-2 cells overexpressing ZeXYP1, AtXYP1 or AtXYP2 accumulate xylogen, as detected by a protein gel blot analysis with anti-ZeXYP1 antibody. Zinnia CM, conditioned medium of Zinnia xylogenic culture. d, Activity of xylogen of transgenic BY-2 cells measured with a Zinnia microbead culture. e, Immunoblotting of conditioned medium (CM) or microsomal fraction (MF) of Zinnia xylogenic culture detected by antibodies against ZeXYP1 (XYP), PIP2a or KNOLLE (KN). f, Selective binding of xylogen to sterols in a protein–sterol overlay assay.

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