Active oxygen species (AOS) generated in response to stimuli and during development can function as signalling molecules in eukaryotes, leading to specific downstream responses1,2. In plants these include such diverse processes as coping with stress (for example pathogen attack3, wounding4 and oxygen deprivation5), abscisic-acid-induced guard-cell closure6, and cellular development (for example root hair growth7). Despite the importance of signalling via AOS in eukaryotes, little is known about the protein components operating downstream of AOS that mediate any of these processes. Here we show that expression of an Arabidopsis thaliana gene (OXI1) encoding a serine/threonine kinase is induced in response to a wide range of H2O2-generating stimuli. OXI1 kinase activity is itself also induced by H2O2 in vivo. OXI1 is required for full activation of the mitogen-activated protein kinases (MAPKs) MPK3 and MPK6 after treatment with AOS or elicitor and is necessary for at least two very different AOS-mediated processes: basal resistance to Peronospora parasitica infection, and root hair growth. Thus, OXI1 is an essential part of the signal transduction pathway linking oxidative burst signals to diverse downstream responses.
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We thank A. Rehmany for the P. parasitica isolates; A. Hailey for statistical analysis; B. Ellis and G. Miles for discussions; and N. Evans, S. Gurr and R. Capper and K. Denby for practical and moral support. This work was funded by the BBSRC, the Gatsby Foundation, the EU TMR program, the Cannon Collins Educational Trust for Southern Africa (CCETSA), the South African National Research Foundation (NRF), and the Austrian Science Foundation.
The authors declare that they have no competing financial interests.
Plants containing an OXI1::GUS gene construct were wounded with tweezers and stained for (a) H2O2 production with diaminobenzidine (DAB) or (b) expression of the GUS protein24. (PDF 236 kb)
Low magnification (top) and higher magnification (bottom) of roots grown on (a) ordinary agar and (b) phytagel. (PDF 132 kb)
Distribution of mature root hair lengths of (a) oxi1 and Ws-2 seedlings and (b) oxi1 and complemented oxi1 seedlings (oxi1 + OXI1) with roots growing through air. (PDF 45 kb)
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Rentel, M., Lecourieux, D., Ouaked, F. et al. OXI1 kinase is necessary for oxidative burst-mediated signalling in Arabidopsis. Nature 427, 858–861 (2004). https://doi.org/10.1038/nature02353
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