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A positive-feedback-based bistable ‘memory module’ that governs a cell fate decision

A Corrigendum to this article was published on 30 August 2007


The maturation of Xenopus oocytes can be thought of as a process of cell fate induction, with the immature oocyte representing the default fate and the mature oocyte representing the induced fate1,2. Crucial mediators of Xenopus oocyte maturation, including the p42 mitogen-activated protein kinase (MAPK) and the cell-division cycle protein kinase Cdc2, are known to be organized into positive feedback loops3. In principle, such positive feedback loops could produce an actively maintained ‘memory’ of a transient inductive stimulus and could explain the irreversibility of maturation3,4,5,6. Here we show that the p42 MAPK and Cdc2 system normally generates an irreversible biochemical response from a transient stimulus, but the response becomes transient when positive feedback is blocked. Our results explain how a group of intrinsically reversible signal transducers can generate an irreversible response at a systems level, and show how a cell fate can be maintained by a self-sustaining pattern of protein kinase activation.

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Figure 1: Cell fate commitment during oocyte maturation.
Figure 2: Irreversibility in the biochemical responses of oocytes to progesterone.
Figure 3: Irreversibility in the biochemical responses of oocytes expressing ΔRaf:ER to oestradiol.
Figure 4: Positive feedback is required for irreversible biochemical responses.


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We thank M. McMahon for the ΔRaf:ER constructs, and K. Cimprich and members of the Ferrell laboratory for discussions and comments on the manuscript. This work was supported by a grant from the NIH.

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Correspondence to James E. Ferrell Jr.

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Xiong, W., Ferrell, J. A positive-feedback-based bistable ‘memory module’ that governs a cell fate decision. Nature 426, 460–465 (2003).

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