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A structural state of the myosin V motor without bound nucleotide

Abstract

The myosin superfamily of molecular motors use ATP hydrolysis and actin-activated product release to produce directed movement and force1. Although this is generally thought to involve movement of a mechanical lever arm attached to a motor core1,2, the structural details of the rearrangement in myosin that drive the lever arm motion on actin attachment are unknown. Motivated by kinetic evidence that the processive unconventional myosin, myosin V, populates a unique state in the absence of nucleotide and actin, we obtained a 2.0 Å structure of a myosin V fragment. Here we reveal a conformation of myosin without bound nucleotide. The nucleotide-binding site has adopted new conformations of the nucleotide-binding elements that reduce the affinity for the nucleotide. The major cleft in the molecule has closed, and the lever arm has assumed a position consistent with that in an actomyosin rigor complex. These changes have been accomplished by relative movements of the subdomains of the molecule, and reveal elements of the structural communication between the actin-binding interface and nucleotide-binding site of myosin that underlie the mechanism of chemo-mechanical transduction.

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Acknowledgements

This work was supported by grants from the National Institutes of Health (H.L.S.), the CNRS and the ARC (A.H.). We thank D. Picot and the staff of the European Synchrotron Radiation Facility for assistance during data collection. We are also grateful to C. Baldacchino and J. Kibbe for help in protein purification.

Author information

Correspondence to H. Lee Sweeney or Anne Houdusse.

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The authors declare that they have no competing financial interests.

Supplementary information

Supplementary Figure 1 (JPG 107 kb)

Supplementary Figure 2 (JPG 81 kb)

Supplementary Figure 3 (JPG 230 kb)

Supplementary Figure Legends and Methods (DOC 30 kb)

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Further reading

Figure 1: Positions of subdomains and connectors in the three myosin states and closure of the 50-kDa cleft.
Figure 2: Nucleotide-binding site and distortion of the β-sheet at the interface of the N-terminal and upper 50-kDa subdomains.
Figure 3: The actin–myosin interface.

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