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Pathogen-induced systemic plant signal triggers DNA rearrangements

Abstract

Plant genome stability is known to be affected by various abiotic environmental conditions1,2,3,4,5,6,7, but little is known about the effect of pathogens. For example, exposure of maize plants to barley stripe mosaic virus seems to activate transposable elements8,9 and to cause mutations in the non-infected progeny of infected plants10. The induction by barley stripe mosaic virus of an inherited effect may mean that the virus has a non-cell-autonomous influence on genome stability. Infection with Peronospora parasitica results in an increase in the frequency of somatic recombination in Arabidopsis thaliana11; however, it is unclear whether effects on recombination require the presence of the pathogen or represent a systemic plant response. It is also not clear whether the changes in the frequency of somatic recombination can be inherited. Here we report a threefold increase in homologous recombination frequency in both infected and non-infected tissue of tobacco plants infected with either tobacco mosaic virus12 or oilseed rape mosaic virus13. These results indicate the existence of a systemic recombination signal that also results in an increased frequency of meiotic and/or inherited late somatic recombination.

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Figure 1: The presence of TMV is not required for the increase in recombination frequency.
Figure 2: Grafting experiments.

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Acknowledgements

We thank S. van Eeden for technical assistance and H. Rothnie, K. Smith and E. Schultz for comments on the manuscript. The Novartis Research Foundation and Alberta Ingenuity Grant are acknowledged for financial support.

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Correspondence to Igor Kovalchuk.

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Kovalchuk, I., Kovalchuk, O., Kalck, V. et al. Pathogen-induced systemic plant signal triggers DNA rearrangements. Nature 423, 760–762 (2003). https://doi.org/10.1038/nature01683

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