Proteomics is the study of the function of all expressed proteins. Tremendous progress has been made in the past few years in generating large-scale data sets for protein–protein interactions, organelle composition, protein activity patterns and protein profiles in cancer patients. But further technological improvements, organization of international proteomics projects and open access to results are needed for proteomics to fulfil its potential.
This is a preview of subscription content
Subscribe to Journal
Get full journal access for 1 year
only $3.90 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Tax calculation will be finalised during checkout.
Get time limited or full article access on ReadCube.
All prices are NET prices.
Wilkins, M. R. et al. From proteins to proteomes: large scale protein identification by two-dimensional electrophoresis and amino acid analysis. Biotechnology 14, 61–65 (1996).
Shoemaker, D. D. & Linsley, P. S. Recent developments in DNA microarrays. Curr. Opin. Microbiol. 5, 334–337 (2002).
Giaever, G. et al. Functional profiling of the Saccharomyces cerevisiae genome. Nature 418, 387–391 (2002).
Gerlai, R. Phenomics: fiction or the future? Trends Neurosci. 25, 506–509 (2002).
Tong, A. H. et al. Systematic genetic analysis with ordered arrays of yeast deletion mutants. Science 294, 2364–2368 (2001).
Hannon, G. J. RNA interference. Nature 418, 244–251 (2002).
Kuruvilla, F. G., Shamji, A. F., Sternson, S. M., Hergenrother, P. J. & Schreiber, S. L. Dissecting glucose signalling with diversity-oriented synthesis and small-molecule microarrays. Nature 416, 653–657 (2002).
Csete, M. E. & Doyle, J. C. Reverse engineering of biological complexity. Science 295, 1664–1669 (2002).
Ficarro, S. B. et al. Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae. Nature Biotechnol. 20, 301–305 (2002).
Liu, H., Lin, D. & Yates, J. R. III Multidimensional separations for protein/peptide analysis in the post-genomic era. Biotechniques 32, 898–911 (2002).
Ideker, T. et al. Integrated genomic and proteomic analyses of a systematically perturbed metabolic network. Science 292, 929–934 (2001).
Fields, S. & Song, O. A novel genetic system to detect protein–protein interactions. Nature 340, 245–246 (1989).
MacBeath, G. Protein microarrays and proteomics. Nature Genet. 32 (Suppl.), 526–532 (2002).
Wright, P. E. & Dyson, H. J. Intrinsically unstructured proteins: re-assessing the protein structure-function paradigm. J. Mol. Biol. 293, 321–331 (1999).
Ashburner, M. et al. Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nature Genet. 25, 25–29 (2000).
Bader, G. D. & Hogue, W. V. C. in Genomics and Bioinformatics (ed. Sensen, C. W.) 399–413 (Wiley-VCH, Weinheim, 2001).
Kitano, H. Systems biology: a brief overview. Science 295, 1662–1664 (2002).
Petricoin, E. F., Zoon, K. C., Kohn, E. C., Barrett, J. C. & Liotta, L. A. Clinical proteomics: translating benchside promise into bedside reality. Nature Rev. Drug Discov. 1, 683–695 (2002).
Andersen, J. S. et al. Directed proteomic analysis of the human nucleolus. Curr. Biol. 12, 1–11 (2002).
Jorgensen, P., Nishikawa, J. L., Breitkreutz, B. J. & Tyers, M. Systematic identification of pathways that couple cell growth and division in yeast. Science 297, 395–400 (2002).
Walhout, A. J. et al. Integrating interactome, phenome, and transcriptome mapping data for the C. elegans germline. Curr. Biol. 12, 1952–1958 (2002).
Manning, B. D., Tee, A. R., Logsdon, M. N., Blenis, J. & Cantley, L. C. Identification of the tuberous sclerosis complex-2 tumor suppressor gene product tuberin as a target of the phosphoinositide 3-kinase/akt pathway. Mol. Cell 10, 151–162 (2002).
Yoon, H. J. et al. Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes. Curr. Biol. 12, 2048–2054 (2002).
Mann, M. & Jensen, O. N. Proteomic analysis of post-translational modifications. Nature Biotechnol. (in the press).
Huang, S. & Ingber, D. E. Shape-dependent control of cell growth, differentiation, and apoptosis: switching between attractors in cell regulatory networks. Exp. Cell Res. 261, 91–103 (2000).
Ball, P. Data visualization: picture this. Nature 418, 11–13 (2002).
Aebersold, R. & Watts, J. D. The need for national centers for proteomics. Nature Biotechnol. 20, 651 (2002).
Marshall, E. Bermuda rules: community spirit, with teeth. Science 291, 1192 (2001).
We thank B.-J. Breitkreutz for preparing Fig. 2, D. Figeys and members of the Center for Experimental BioInformatics (CEBI) for critical reading of the manuscript. CEBI is supported by a grant from the Danish Natural Research Foundation.
About this article
Cite this article
Tyers, M., Mann, M. From genomics to proteomics. Nature 422, 193–197 (2003). https://doi.org/10.1038/nature01510
Inflammation and remodeling pathways and risk of cardiovascular events in patients with ischemic heart failure and reduced ejection fraction
Scientific Reports (2022)
Current Treatment Options in Oncology (2022)
iTRAQ-based quantitative proteomic analysis of thoracic aortas from adult rats born to preeclamptic dams
Clinical Proteomics (2021)
Polypropylene capillary-channeled polymer fiber column as the second dimension in a comprehensive two-dimensional RP × RP analysis of a mixture of intact proteins
Analytical and Bioanalytical Chemistry (2020)