Free fatty acids regulate insulin secretion from pancreatic β cells through GPR40

Abstract

Diabetes, a disease in which carbohydrate and lipid metabolism are regulated improperly by insulin, is a serious worldwide health issue1,2. Insulin is secreted from pancreatic β cells in response to elevated plasma glucose, with various factors modifying its secretion3. Free fatty acids (FFAs) provide an important energy source as nutrients, and they also act as signalling molecules in various cellular processes, including insulin secretion4,5. Although FFAs are thought to promote insulin secretion in an acute phase, this mechanism is not clearly understood6. Here we show that a G-protein-coupled receptor, GPR40, which is abundantly expressed in the pancreas, functions as a receptor for long-chain FFAs. Furthermore, we show that long-chain FFAs amplify glucose-stimulated insulin secretion from pancreatic β cells by activating GPR40. Our results indicate that GPR40 agonists and/or antagonists show potential for the development of new anti-diabetic drugs.

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Figure 1: GPR40 mRNA is expressed abundantly in pancreatic β cells.
Figure 2: Induction of [Ca2+]i rise and MAP kinase activation by FFAs in CHO–hGPR40 cells.
Figure 3: Analyses for FFA-induced insulin secretion mechanisms.

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Acknowledgements

We thank Y. Sumino, T. Soda, K. Kato, H. Odaka, F. Itoh, M. Kajino, K. Fukatsu and N. Suzuki for their discussions.

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Correspondence to Shuji Hinuma.

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The authors declare that they have no competing financial interests.

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Itoh, Y., Kawamata, Y., Harada, M. et al. Free fatty acids regulate insulin secretion from pancreatic β cells through GPR40. Nature 422, 173–176 (2003). https://doi.org/10.1038/nature01478

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