Growth dynamics underlying petal shape and asymmetry

Abstract

Development commonly involves the generation of complex shapes from simpler ones. One way of following this process is to use landmarks to track the fate of particular points in a developing organ1,2,3,4,5,6,7, but this is limited by the time over which it can be monitored. Here we use an alternative method, clonal analysis8, whereby dividing cells are genetically marked and their descendants identified visually, to observe the development of Antirrhinum (snapdragon) petals. Clonal analysis has previously been used to estimate growth parameters of leaves9,10,11 and Drosophila wings12,13,14 but these results were not integrated within a dynamic growth model. Here we develop such a model and use it to show that a key aspect of shape—petal asymmetry—in the petal lobe of Antirrhinum depends on the direction of growth rather than regional differences in growth rate. The direction of growth is maintained parallel to the proximodistal axis of the flower, irrespective of changes in shape, implying that long-range signals orient growth along the petal as a whole. Such signals may provide a general mechanism for orienting growth in other growing structures.

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Figure 1: Determining growth parameters.
Figure 2: Results and validation of growth analysis.
Figure 3: Petal lobe shapes at P46 resulting from growth simulations run forwards in developmental time from P32.
Figure 4: Growth of petal lobe in relation to the whole flower.

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Acknowledgements

We thank R. Carpenter for providing plant stocks, C. Vincent and K. Lee for the scanning electron microscopy, J. Dumais for help with SEM 3D reconstructions, N. Orme for drawings in Fig. 1, S. J. Impey for gathering the image database and for early work on the project, supported by a BBSRC grant. A.-G. R.-L. was supported by a Norwich Research Park studentship.

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Correspondence to Enrico Coen.

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Rolland-Lagan, A., Bangham, J. & Coen, E. Growth dynamics underlying petal shape and asymmetry. Nature 422, 161–163 (2003). https://doi.org/10.1038/nature01443

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