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Functional and spatial segregation of secretory vesicle pools according to vesicle age

Abstract

Synaptic terminals and neuroendocrine cells are packed with secretory vesicles, only a few of which are docked at the plasma membrane and readily releasable. The remainder are thought to constitute a large cytoplasmic reserve pool awaiting recruitment into the readily releasable pool (RRP) for exocytosis1. How vesicles are prioritized in recruitment is still unknown: the choice could be random, or else the oldest or the newest ones might be favoured. Here we show, using a fluorescent cargo protein2 that changes colour with time3, that vesicles in bovine adrenal chromaffin cells segregate into distinct populations, based on age. Newly assembled vesicles are immobile (morphologically docked) at the plasma membrane shortly after biogenesis, whereas older vesicles are mobile and located deeper in the cell. Different secretagogues selectively release vesicles from the RRP or, surprisingly, selectively from the deeper cytoplasmic pool. Thus, far from being equal, vesicles are segregated functionally and spatially according to age.

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Figure 1: ANF–dsRed-E5-transduced chromaffin cells contain populations of LDCVs with distinct colours and distributions.
Figure 2: TIRFM imaging of transduced living BCCs resolves a membrane-proximal pool of labelled vesicles.
Figure 3: The mean squared displacement (〈d2〉) was calculated for individual LDCVs from each ANF–dsRed-E5-coloured population, revealing differing mobilities for each group.

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Acknowledgements

We thank A. Morgan for the catecholamine assay protocol; J. Pryde for the polyclonal anti-Golgi serum; D. J. Webb and N. Johnston for the anti-ANF serum and RIA protocol; and L. Sharp for assistance with confocal microscopy. This work was funded by Wellcome Trust grants and an NIH grant to D.K.A., R.H.C. and M.J.S.

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Correspondence to Michael J. Shipston or Robert H. Chow.

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Duncan, R., Greaves, J., Wiegand, U. et al. Functional and spatial segregation of secretory vesicle pools according to vesicle age. Nature 422, 176–180 (2003). https://doi.org/10.1038/nature01389

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