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Auxin promotes Arabidopsis root growth by modulating gibberellin response

Abstract

The growth of plant organs is influenced by a stream of the phytohormone auxin that flows from the shoot apex to the tip of the root1. However, until now it has not been known how auxin regulates the cell proliferation and enlargement that characterizes organ growth. Here we show that auxin controls the growth of roots by modulating cellular responses to the phytohormone gibberellin (GA). GA promotes the growth of plants by opposing the effects of nuclear DELLA protein growth repressors2,3,4,5,6,7,8, one of which is Arabidopsis RGA (for repressor of gal-3)9,10. GA opposes the action of several DELLA proteins by destabilizing them, reducing both the concentration of detectable DELLA proteins and their growth-restraining effects9,10,11,12,13,14. We also show that auxin is necessary for GA-mediated control of root growth, and that attenuation of auxin transport or signalling delays the GA-induced disappearance of RGA from root cell nuclei. Our observations indicate that the shoot apex exerts long-distance control on the growth of plant organs through the effect of auxin on GA-mediated DELLA protein destabilization.

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Figure 1: GA regulates root elongation via DELLA proteins.
Figure 2: Shoot apex-derived auxin controls root growth.
Figure 3: NPA inhibits the effect of GA on root growth and disappearance of GFP–RGA from root cell nuclei.
Figure 4: Decrease in AtPIN1 or AXR1 function delays GA-induced disappearance of GFP–RGA.

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Acknowledgements

We thank T.-p. Sun (Duke University) for the pRGA:GFPRGA line, O. Leyser for axr1-12, G. Calder for assistance with confocal microscopy, and P. Achard, T. Ait-ali, E. Coen, O. Leyser, D. Richards and X. Zhang for comments on the manuscript. Funding was received from the BBSRC (Core Strategic Grant to the John Innes Centre and a response mode grant).

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Correspondence to Nicholas P. Harberd.

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Fu, X., Harberd, N. Auxin promotes Arabidopsis root growth by modulating gibberellin response. Nature 421, 740–743 (2003). https://doi.org/10.1038/nature01387

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