In the mouse circadian clock, a transcriptional feedback loop is at the centre of the clockwork mechanism. Clock and Bmal1 are essential transcription factors that drive the expression of three period genes (Per1–3) and two cryptochrome genes (Cry1 and Cry2)1,2,3,4,5. The Cry proteins feedback to inhibit Clock/Bmal1-mediated transcription by a mechanism that does not alter Clock/Bmal1 binding to DNA6. Here we show that transcriptional regulation of the core clock mechanism in mouse liver is accompanied by rhythms in H3 histone acetylation, and that H3 acetylation is a potential target of the inhibitory action of Cry. The promoter regions of the Per1, Per2 and Cry1 genes exhibit circadian rhythms in H3 acetylation and RNA polymerase II binding that are synchronous with the corresponding steady-state messenger RNA rhythms. The histone acetyltransferase p300 precipitates together with Clock in vivo in a time-dependent manner. Moreover, the Cry proteins inhibit a p300-induced increase in Clock/Bmal1-mediated transcription. The delayed timing of the Cry1 mRNA rhythm, relative to the Per rhythms, is due to the coordinated activities of Rev-Erbα and Clock/Bmal1, and defines a new mechanism for circadian phase control.
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We thank D. Reinberg for the anti-RPB1 antibody; V. Sartorelli for the p300 expression construct; U. Schibler for Rev-Erbα reagents; H. R. Ueda for PATSER sequence analysis; and D. R. Weaver and C. L. Peterson for suggestions. This work was supported by grants from the NIH and the Defense Advanced Research Projects Agency (DARPA).
The authors declare that they have no competing financial interests.
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Etchegaray, JP., Lee, C., Wade, P. et al. Rhythmic histone acetylation underlies transcription in the mammalian circadian clock. Nature 421, 177–182 (2003). https://doi.org/10.1038/nature01314
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