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Targeted recycling of PECAM from endothelial surface-connected compartments during diapedesis

Abstract

Leukocytes enter sites of inflammation by squeezing through the borders between endothelial cells that line postcapillary venules at that site. This rapid process, called transendothelial migration (TEM) or diapedesis, is completed within 90 s after a leukocyte arrests on the endothelial surface1,2,3,4. In this time, the leukocyte moves in ameboid fashion across the endothelial borders, which remain tightly apposed to it during transit. It is not known how the endothelial cell changes its borders rapidly and reversibly to accommodate the migrating leukocyte. Here we show that there is a membrane network just below the plasmalemma at the cell borders that is connected at intervals to the junctional surface. PECAM-1, an integral membrane protein with an essential role in TEM5,6,7, is found in this compartment and constitutively recycles evenly along endothelial cell borders. During TEM, however, recycling PECAM is targeted to segments of the junction across which monocytes are in the act of migration. In addition, blockade of TEM with antibodies against PECAM specifically blocks the recruitment of this membrane to the zones of leukocyte migration, without affecting the constitutive membrane trafficking.

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Acknowledgements

We thank R. Liebman for technical assistance; P. Newman for the P1.1 antibody; and P. Brennwald, T. McGraw and T. Ryan for discussions and comments on the manuscript. Supported by NIH grants (to W.A.M. and F.R.M.), a Charles H. Revson Foundation Fellowship (to Z.M.), and an Atorvastatin Research Award from Pfizer/Parke Davis (to L.P.).

Author information

Correspondence to William A. Muller.

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The authors declare that they have no competing financial interests.

Supplementary information

Supplementary Figure 1S (JPG 82 kb)

Supplementary Figure 2S (JPG 88 kb)

Supplementary Figure 3S (JPG 12 kb)

Supplementary Figure 4S (JPG 42 kb)

Supplementary Figure 5S (PDF 479 kb)

Supplementary Table 1, Materials, Methods, Analysis and Figure Legends (DOC 46 kb)

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Further reading

Figure 1: PECAM-rich membrane is rapidly internalized from the cell border into a subjunctional reticulum.
Figure 2: PECAM-bearing membrane enters a reticulum of surface-connected compartments.
Figure 3: Quantification of PECAM recycling.
Figure 4: Recycling PECAM is involved in transendothelial migration.

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