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Variable effects of nitrogen additions on the stability and turnover of soil carbon


Soils contain the largest near-surface reservoir of terrestrial carbon1 and so knowledge of the factors controlling soil carbon storage and turnover is essential for understanding the changing global carbon cycle. The influence of climate on decomposition of soil carbon has been well documented2,3, but there remains considerable uncertainty in the potential response of soil carbon dynamics to the rapid global increase in reactive nitrogen (coming largely from agricultural fertilizers and fossil fuel combustion). Here, using 14C, 13C and compound-specific analyses of soil carbon from long-term nitrogen fertilization plots, we show that nitrogen additions significantly accelerate decomposition of light soil carbon fractions (with decadal turnover times) while further stabilizing soil carbon compounds in heavier, mineral-associated fractions (with multidecadal to century lifetimes). Despite these changes in the dynamics of different soil pools, we observed no significant changes in bulk soil carbon, highlighting a limitation inherent to the still widely used single-pool approach to investigating soil carbon responses to changing environmental conditions. It remains to be seen if the effects observed here—caused by relatively high, short-term fertilizer additions—are similar to those arising from lower, long-term additions of nitrogen to natural ecosystems from atmospheric deposition, but our results suggest nonetheless that current models of terrestrial carbon cycling do not contain the mechanisms needed to capture the complex relationship between nitrogen availability and soil carbon storage.

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Figure 1: 14C activity of Niwot Ridge plant material compared to atmospheric trends13.


  1. Post, W. M., Emanuel, W. R., Zinke, P. J. & Stangenberger, A. G. Soil carbon pools and world life zones. Nature 298, 156–159 (1982)

    ADS  CAS  Article  Google Scholar 

  2. Trumbore, S. E., Chadwick, O. A. & Amundson, R. Rapid exchange between soil carbon and atmospheric carbon dioxide driven by temperature change. Science 272, 393–396 (1996)

    ADS  CAS  Article  Google Scholar 

  3. Epstein, H. E., Burke, I. C. & Lauenroth, W. K. Regional patterns of decomposition and primary production rates in the US Great Plains. Ecology 83(2), 320–327 (2002)

    Google Scholar 

  4. Vitousek, P. M. et al. Human alteration of the global nitrogen cycle: sources and consequences. Ecol. Appl. 7(3), 737–750 (1997)

    Google Scholar 

  5. Townsend, A. R., Braswell, B. H., Holland, E. A. & Penner, J. E. Spatial and temporal patterns in terrestrial carbon storage due to deposition of fossil fuel nitrogen. Ecol. Appl. 6(3), 806–814 (1996)

    Article  Google Scholar 

  6. Vitousek, P. M. Nutrient cycling and nutrient use efficiency. Am. Nat. 119, 553–572 (1982)

    Article  Google Scholar 

  7. Melillo, J. M. & Aber, J. D. Nitrogen and lignin control of hardwood leaf litter decomposition dynamics. Ecology 63(3), 621–626 (1982)

    Article  Google Scholar 

  8. Hobbie, S. E. Interactions between litter lignin and soil nitrogen availability during leaf litter decomposition in a Hawaiian montane forest. Ecosystems 3, 484–494 (2000)

    CAS  Article  Google Scholar 

  9. Resh, S. C., Binkely, D. & Parrotta, J. A. Greater soil carbon sequestration under nitrogen-fixing trees compared with Eucalyptus species. Ecosystems 5, 217–231 (2002)

    CAS  Article  Google Scholar 

  10. Berg, B. & Matzner, E. Effect of N deposition on decomposition of plant litter and soil organic matter in forest systems. Environ. Rev. 5, 1–25 (1997)

    CAS  Article  Google Scholar 

  11. Bowman, W. D., Theodose, T. A., Schardt, J. C. & Conant, R. T. Constraints of nutrient availability on primary production in two alpine communities. Ecology 74, 2085–2098 (1993)

    Article  Google Scholar 

  12. Schlesinger, W. H. & Lichter, J. Limited carbon storage in soil and litter of experimental forest plots under increased CO2 . Nature 411, 466–468 (2001)

    ADS  CAS  Article  Google Scholar 

  13. Magill, A. H. et al. Biogeochemical response of forest ecosystems to stimulated chronic nitrogen deposition. Ecol. Appl. 7(2), 402–415 (1997)

    Article  Google Scholar 

  14. Levin, I. & Kromer, B. Trends: A Compendium of Data on Global Change (Carbon Dioxide Information Analysis Center, Oak Ridge National Laboratory, US DOE, Oak Ridge, TN, 1997)

    Google Scholar 

  15. Trumbore, S. E. Comparison of carbon dynamics in tropical and temperate soils using radiocarbon measurements. Glob. Biogeochem. Cycles 7(2), 275–290 (1993)

    ADS  Article  Google Scholar 

  16. Gaudinski, J. B., Trumbore, S. E., Davidson, E. A. & Zheng, S. Soil carbon cycling in a temperate forest: radiocarbon-based estimates of residence times, sequestration rates and partitioning of fluxes. Biogeochemistry 51(1), 33–69 (2000)

    Article  Google Scholar 

  17. Gleixner, G., Poirier, N., Bol, R. & Balesdent, J. Molecular dynamics of organic matter in a cultivated soil. Org. Geochem. 33(3), 357–366 (2002)

    Article  Google Scholar 

  18. Nierop, K. G. J., Pulleman, M. M. & Marinissen, J. C. Y. Management induced organic matter differentiation in grassland and arable soil: a study using pyrolysis techniques. Soil Biol. Biochem. 33, 755–764 (2001)

    CAS  Article  Google Scholar 

  19. Kuder, T. & Kruge, M. A. Preservation of biomolecules in sub-fossil plants from raised peat bogs—a potential paleoenvironmental proxy. Org. Geochem. 29(5–7), 1355–1368 (1998)

    Article  Google Scholar 

  20. Schmidt, H. L. & Gleixner, G. Stable Isotopes. Integration of Biological, Ecological and Geochemical Processes (ed. Griffith, H.) 13–25 (BIOS, Oxford, UK, 1998)

    Google Scholar 

  21. Schimel, D. S. et al. Climatic, edaphic and biotic controls over storage and turnover of carbon in soils. Glob. Biogeochem. Cycles 8(3), 279–293 (1994)

    ADS  Article  Google Scholar 

  22. Aber, J. et al. Nitrogen saturation in temperate forest ecosystems—hypotheses revisited. Bioscience 48(11), 921–934 (1998)

    Article  Google Scholar 

  23. Burns, S. F. Alpine Soil Distribution and Development, Indian Peaks, Colorado Front Range. PhD Univ. Colorado (1980)

    Google Scholar 

  24. Williams, M. W., Hood, E. & Caine, N. Role of organic nitrogen in the nitrogen cycle of a high elevation catchment, Colorado Front Range. Wat. Resour. Res. 37(10), 2569–2581 (2001)

    ADS  Article  Google Scholar 

  25. Stuiver, M. & Polach, H. A. Discussion: Reporting of 14C data. Radiocarbon 19(3), 355–363 (1977)

    Article  Google Scholar 

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We thank D. Schimel, S. Hobbie, J. Harden, T. Seastedt and C. Cleveland for comments on an earlier draft of this manuscript. This work was supported by the Andrew Mellon Foundation, the NSF-LTER program, the US Geological Survey and the Max Planck Institute for Biogeochemistry.

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Correspondence to Jason C. Neff.

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Neff, J., Townsend, A., Gleixner, G. et al. Variable effects of nitrogen additions on the stability and turnover of soil carbon. Nature 419, 915–917 (2002).

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