The human malaria parasite Plasmodium falciparum is responsible for the death of more than a million people every year1. To stimulate basic research on the disease, and to promote the development of effective drugs and vaccines against the parasite, the complete genome of P. falciparum clone 3D7 has been sequenced, using a chromosome-by-chromosome shotgun strategy2,3,4. Here we report the nucleotide sequence of the third largest of the parasite's 14 chromosomes, chromosome 12, which comprises about 10% of the 23-megabase genome. As the most (A + T)-rich (80.6%) genome sequenced to date, the P. falciparum genome presented severe problems during the assembly of primary sequence reads. We discuss the methodology that yielded a finished and fully contiguous sequence for chromosome 12. The biological implications of the sequence data are more thoroughly discussed in an accompanying Article (ref. 3).
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We acknowledge the generosity of the participating scientists at Stanford University, TIGR, the WTSI, the NMRC and Oxford University. We also thank N. Hall, M. Berriman, A. Pain and B. Barrell for their time and expertise during the gene-calling annotation process, and are grateful to the members of our Stanford Genome Technology Center for their assistance throughout this project. We thank the Burroughs Wellcome Fund for support that allowed us to participate in the international Malaria Genome Project.
The authors declare that they have no competing financial interests.
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