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Robustness of the BMP morphogen gradient in Drosophila embryonic patterning

Abstract

Developmental patterning relies on morphogen gradients, which generally involve feedback loops to buffer against perturbations caused by fluctuations in gene dosage and expression1. Although many gene components involved in such feedback loops have been identified, how they work together to generate a robust pattern remains unclear. Here we study the network of extracellular proteins that patterns the dorsal region of the Drosophila embryo by establishing a graded activation of the bone morphogenic protein (BMP) pathway. We find that the BMP activation gradient itself is robust to changes in gene dosage. Computational search for networks that support robustness shows that transport of the BMP class ligands (Scw and Dpp) into the dorsal midline by the BMP inhibitor Sog is the key event in this patterning process. The mechanism underlying robustness relies on the ability to store an excess of signalling molecules in a restricted spatial domain where Sog is largely absent. It requires extensive diffusion of the BMP–Sog complexes, coupled with restricted diffusion of the free ligands. We show experimentally that Dpp is widely diffusible in the presence of Sog but tightly localized in its absence, thus validating a central prediction of our theoretical study.

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Figure 1: Robustness of the pMad activation profile.
Figure 2: Patterning mechanism emerges from the features of the robust networks.
Figure 3: Properties of the robust model.
Figure 4: Dpp diffusion requires Sog and Tsg.

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Acknowledgements

We thank P. ten Dijke for the pMad antibody; L. Marsh, M. O'Connor, S. Roth, D. St. Johnston and the Umea and Bloomington Fly Centers for strains; and S. Leibler and S. Roth for comments and criticism. This work was funded by the Israel Science Foundation (B-Z.S.) and the Israel Science Foundation and the Minerva Foundation (N.B). H.A. is a Lister Institute Research Fellow. B-Z.S. is the incumbent of the Hilda and Cecil Lewis professorial chair in Molecular Genetics. N.B. is the incumbent of the Soretta and Henry Shapiro career development chair.

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Correspondence to Naama Barkai.

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Eldar, A., Dorfman, R., Weiss, D. et al. Robustness of the BMP morphogen gradient in Drosophila embryonic patterning. Nature 419, 304–308 (2002). https://doi.org/10.1038/nature01061

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