Directionally selective calcium signals in dendrites of starburst amacrine cells

Abstract

The detection of image motion is fundamental to vision. In many species, unique classes of retinal ganglion cells selectively respond to visual stimuli that move in specific directions. It is not known which retinal cell first performs the neural computations that give rise to directional selectivity in the ganglion cell. A prominent candidate has been an interneuron called the ‘starburst amacrine cell’. Using two-photon optical recordings of intracellular calcium concentration, here we find that individual dendritic branches of starburst cells act as independent computation modules. Dendritic calcium signals, but not somatic membrane voltage, are directionally selective for stimuli that move centrifugally from the cell soma. This demonstrates that direction selectivity is computed locally in dendritic branches at a stage before ganglion cells.

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Figure 1: Calcium responses in starburst amacrine cell dendrites.
Figure 2: Surround stimulation evokes a decrease in [Ca2+] and occasionally transient increases after the end of the stimulus.
Figure 3: Local stimulation evokes local calcium signals.
Figure 4: Moving stimuli evoke direction-selective calcium responses in dendrites.
Figure 5: Preferred direction and cellular morphology.
Figure 6: The GABAA blocker SR-95531 caused increased spontaneous activity but no reduction of [Ca2+]-signal direction selectivity.
Figure 7: Responses to expanding and contracting waves.

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Acknowledgements

We thank A. Borst and D. Margolis for useful discussions, R. Friedrich for comments on the manuscript, and M. Müller, J. Tritthart and J. Sawinski for technical help.

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Correspondence to Thomas Euler.

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W.D. is a co-holder of a patent for two-photon microscopy.

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Euler, T., Detwiler, P. & Denk, W. Directionally selective calcium signals in dendrites of starburst amacrine cells. Nature 418, 845–852 (2002). https://doi.org/10.1038/nature00931

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