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Pluripotency of mesenchymal stem cells derived from adult marrow

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  • A Corrigendum to this article was published on 14 June 2007

Abstract

We report here that cells co-purifying with mesenchymal stem cells—termed here multipotent adult progenitor cells or MAPCs—differentiate, at the single cell level, not only into mesenchymal cells, but also cells with visceral mesoderm, neuroectoderm and endoderm characteristics in vitro. When injected into an early blastocyst, single MAPCs contribute to most, if not all, somatic cell types. On transplantation into a non-irradiated host, MAPCs engraft and differentiate to the haematopoietic lineage, in addition to the epithelium of liver, lung and gut. Engraftment in the haematopoietic system as well as the gastrointestinal tract is increased when MAPCs are transplanted in a minimally irradiated host. As MAPCs proliferate extensively without obvious senescence or loss of differentiation potential, they may be an ideal cell source for therapy of inherited or degenerative diseases.

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Acknowledgements

The authors wish to thank M. Jenkins for technical support. This work was supported by NIH grants, the Michael J. Fox Foundation, the Children's Cancer Research Fund, the Tulloch Family Foundation, and the McKnight Foundation. R.E.S., C.D.K., X.R.O.-G. and M.R. are supported by the NIH-MSTP programme at the University of Minnesota.

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Competing interests

C.M.V. is a consultant for MCL low liability company, Minneapolis.

Supplementary information

  1. Supplementary Information Figure 1 (JPG 412 kb)

  2. Supplementary Information Figure 2 (JPG 207 kb)

  3. Supplementary Information Figure 3 (JPG 205 kb)

  4. Supplementary Information Figure 4 (JPG 246 kb)

  5. Supplementary Information Table 1: Selection and culture of murine MAPC (DOC 72 kb)

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DOI

https://doi.org/10.1038/nature00870

Further reading

Figure 1: Characteristics of mMAPCs.
Figure 2: Single cell origin of mMAPC and rMAPC cultures initiated from ten cells per well, and their differentiated progeny (see also Supplementary Information Table 2).
Figure 3: In vitro differentiation of mMAPCs to endothelium, neuroectoderm and endoderm.
Figure 4: Chimaerism detection by X-gal staining and anti-β-gal staining in animals generated from blastocysts microinjected with a single ROSA26 MAPC (see also Table 1 and Supplementary Information Fig. 4).
Figure 5: Immunofluorescence staining of individual organs from a 45% chimaeric mouse.
Figure 6: Engraftment and in vivo differentiation of mMAPCs.

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