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DNA nanotechnology

Nature Reviews Materials volume 3, Article number: 17068 (2018) Cite this article

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Abstract

DNA is the molecule that stores and transmits genetic information in biological systems. The field of DNA nanotechnology takes this molecule out of its biological context and uses its information to assemble structural motifs and then to connect them together. This field has had a remarkable impact on nanoscience and nanotechnology, and has been revolutionary in our ability to control molecular self-assembly. In this Review, we summarize the approaches used to assemble DNA nanostructures and examine their emerging applications in areas such as biophysics, diagnostics, nanoparticle and protein assembly, biomolecule structure determination, drug delivery and synthetic biology. The introduction of orthogonal interactions into DNA nanostructures is discussed, and finally, a perspective on the future directions of this field is presented.

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Figure 1: A timeline of the field of DNA nanotechnology.
Figure 2: The beginning of DNA nanotechnology.
Figure 3: DNA origami and single-stranded tile assembly.
Figure 4: Three-dimensional structures from DNA.
Figure 5: Dynamic DNA nanostructures.
Figure 6: Supramolecular DNA assembly.
Figure 7: Interaction of DNA structures with polymers and lipids.
Figure 8: Nanoparticle assembly with DNA.
Figure 9: Protein assembly with DNA.
Figure 10: Biological applications of DNA nanotechnology.

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Acknowledgements

This research has been supported by the following grants to H.F.S.: Natural Sciences and Engineering Research Council of Canada (NSERC), Canada Research Chairs Program, the Canadian Institutes of Health Research (CIHR), Fonds de recherche du Québec — Nature et technologies (FRQNT) and Prostate Cancer Canada. The following grants were provided to N.C.S.: EFRI-1332411 and CCF-1526650 from the National Science Foundation (NSF), MURI W911NF-11-1-0024 from the US Army Research Office (ARO), N000141110729 from the US Office of Naval Research (ONR), DE-SC0007991 from the US Department of Energy (DOE) for DNA synthesis, and partial salary support and grant GBMF3849 from the Gordon and Betty Moore Foundation. The authors thank P. Chidchob, A. Lacroix, N. Avakyan, J. Hsu, D. Bousmail, T. Trinh, D. de Rochambeau, H. Fakih, E. Vengut Climent and M. Dore for help proofreading the manuscript.

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  1. Department of Chemistry, New York University, 100 Washington Square East, New York, 10003, USA

    Nadrian C. Seeman

  2. Department of Chemistry, McGill University, 801 Sherbrooke St. West, Montréal, Québec, H3A0B8, Canada

    Hanadi F. Sleiman

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H.F.S. researched the data for the article. H.F.S. and N.C.S. wrote the article and edited it before submission.

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Seeman, N., Sleiman, H. DNA nanotechnology. Nat Rev Mater 3, 17068 (2018). https://doi.org/10.1038/natrevmats.2017.68

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