Figure 2

From: A paternal methyl donor-rich diet altered cognitive and neural functions in offspring mice

Figure 2

Hippocampal Kcnmb2 overexpression improved water maze impairments in MD F1 mice. (a) Experimental design. (b) Representative image of hippocampal slices infected with AAV1-hSyn1-Kcnmb2-IRES-GFP or control AAV1-hSyn1-GFP virus. Green, GFP, imaged 4 weeks after virus injection. Scale bar, 100 μm. (c) Relative Kcnmb2 mRNA expression in the hippocampus after viral infection as determined by qPCR (n=5 mice per group; two-way ANOVA with the between-subjects factors paternal diet and AAV treatment: effect of paternal diet, P=0.0214; effect of AAV treatment, P<0.0001; paternal diet × AAV treatment interaction, P=0.7084). (d and e) Quadrant occupancy measures (d) and target crossings (e) during a probe trial given after completion of water maze training (CD F1 control-AAV, n=12 mice; MD F1 control-AAV, n=12 mice; CD F1 Kcnmb2-AAV, n=8 mice; MD F1 Kcnmb2-AAV, n=8 mice). Pool quadrants: TQ; AR; OQ; and AL. All graphs display mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001. AL, adjacent left; ANOVA, analysis of variance; AR, adjacent right; CD, control diet; GFP, green fluorescent protein; MD, methyl donor-rich diet; OQ, opposite quadrant; TQ, target quadrant.