Figure 1

From: A paternal methyl donor-rich diet altered cognitive and neural functions in offspring mice

Figure 1

Impaired hippocampus-dependent learning and memory and altered hippocampal functions in MD F1 offspring mice. (a) Experimental design. (b and c) Quadrant occupancy measures (b) and target crossings (c) during a probe trial given after completion of water maze training (n=20 mice per group). Pool quadrants: TQ; AR; OQ; and AL. (d) Freezing during a context test given 1 day after training in a contextual fear conditioning paradigm (CD F1: n=17 mice; MD F1: n=14 mice). (e) Measurements of long-term potentiation at Schaffer Collateral/CA1 synapses in acute brain slices (CD F1, n=7 mice; MD F1, n=8 mice). (f) Quantification of oscillatory activity in the theta range measured during video-EEG analysis of CD F1 and MD F1 mice (n=11 mice per group). (g) Representative time frequency plots show reduced typical theta segments in a MD F1 mouse relative to a CD F1 mouse. All graphs display mean ± s.e.m. *P<0.05, **P<0.01, ***P<0.001. AL, adjacent left; AR, adjacent right; CD, control diet; EEG, electroencephalogram; MD, methyl donor-rich diet; OQ, opposite quadrant; TQ, target quadrant.