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Metabolic mapping of downstream network activity following CNO-induced activation of hM3Dq in BNST VGAT neurons

Molecular Psychiatry volume 23, page 1 (2018)Cite this article

(a) Injection of a DIO-mCherry to the BNST of a VGAT-Cre mouse to label projection fibers. (b) mCherry fluorescence observed in the BNST injection site (left) and fluorescent fibers in the VTA (middle) and PBN/LC (right). Scale bars: 1 mm. (c) μPET imaging timeline. Mice were injected with vehicle or CNO and 5 min later injected with FDG. Twenty-five minutes later, mice were anesthetized with isoflurane and placed on the scanning bed for a 20 min scanning session. (d) Representative image of a DIO-hM3Dq-mCherry BNST injection site. Scale bar: 500 μm. (e) Increased FDG uptake in areas corresponding to the BNST (left), VTA (middle) and PBN/LC (right) following activation of hM3Dq in BNST VGAT neurons. (f) Representative injection of a DIO-hM3Dq-mCherry to a Cre-negative control mouse. (g) No change in FDG uptake in the BNST (left), VTA (middle), or PBN/LC (right) from pooled adjacent controls. a.c., anterior commissure; BNST, bed nucleus of the stria terminalis; LC, locus coeruleus; N, four mice per group; PBN, parabrachial nucleus; VTA, ventral tegmental area. For more information on this topic, please refer to the article by Mazzone et al. on pages 143–153.

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Authors and Affiliations

  1. Neurobiology Curriculum, University of North Carolina, Chapel Hill, NC, USA

    C M Mazzone

  2. Laboratory of Molecular Neurophysiology, Bowles Center for Alcohol Studies, University of North Carolina School of Medicine, Chapel Hill, NC, USA

    C M Mazzone, D Pati, J DiBerto, G Tipton, K Duffy, J M McKlveen, J A Hardaway & T L Kash

  3. Fishberg Department of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    M Michaelides & Y L Hurd

  4. Department of Psychiatry, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA

    M Michaelides & Y L Hurd

  5. Biobehavioral Imaging and Molecular Neuropsychopharmacology Unit, Neuroimaging Research Branch, National Institute on Drug Abuse, Baltimore, MD, USA

    M Michaelides

  6. Department of Psychology, University of Vermont, Burlington, VT, USA

    J H Fox, W A Falls & S E Hammack

  7. Center for Gastrointestinal Biology and Disease, University of North Carolina, Chapel Hill, NC, USA

    C Anderson

  8. Department of Medicine, University of North Carolina, Chapel Hill, NC, USA

    S T Magness

  9. Department of Cell Biology and Physiology, and Biomedical Engineering, University of North Carolina, Chapel Hill, NC, USA

    S T Magness

  10. Department of Psychiatry, University of North Carolina, Chapel Hill, NC, USA

    Z A McElligott

  11. Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    T L Kash

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  1. C M Mazzone
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Mazzone, C., Pati, D., Michaelides, M. et al. Metabolic mapping of downstream network activity following CNO-induced activation of hM3Dq in BNST VGAT neurons. Mol Psychiatry 23, 1 (2018). https://doi.org/10.1038/mp.2017.253

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