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Amphetamine-induced behavior requires CaMKII-dependent dopamine transporter phosphorylation

Molecular Psychiatry volume 19, pages 279–281 (2014)Cite this article

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The dopamine (DA) transporter (DAT), which mediates the inactivation of released DA through its reuptake, is a primary molecular target for psychostimulants.1, 2 Cocaine and methylphenidate (MPH) exert their psychostimulant properties by blocking DA reuptake, leading to the elevation of extracellular DA.2 In contrast, amphetamine (AMPH) acts as a substrate for DAT and subsequently induces non-exocytotic DAT-mediated release of DA (DA efflux).2 Here we use a Drosophila behavioral assay to delineate the signaling mechanisms that modulate DAT-mediated AMPH-induced behavior in vivo. Understanding these mechanisms is critical to understanding how the actions of AMPH might be blocked therapeutically, while simultaneously preserving DA transport.

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Authors and Affiliations

  1. Center for Molecular Recognition, Columbia University College of Physicians and Surgeons, New York, NY, USA

    A B Pizzo, C S Karam, Y Zhang, C L Ma & J A Javitch

  2. Department of Psychiatry, Columbia University College of Physicians and Surgeons, New York, NY, USA

    A B Pizzo, C S Karam, Y Zhang & J A Javitch

  3. Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, NY, USA

    A B Pizzo, C S Karam, Y Zhang & J A Javitch

  4. Barnard College, New York, NY, USA

    C L Ma

  5. Department of Pathology and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY, USA

    B D McCabe

  6. Department of Neuroscience, Columbia University College of Physicians and Surgeons, New York, NY, USA

    B D McCabe

  7. Department of Pharmacology, Columbia University College of Physicians and Surgeons, New York, NY, USA

    J A Javitch

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  1. A B Pizzo
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  2. C S Karam
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  3. Y Zhang
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  4. C L Ma
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  5. B D McCabe
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Correspondence to J A Javitch.

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Pizzo, A., Karam, C., Zhang, Y. et al. Amphetamine-induced behavior requires CaMKII-dependent dopamine transporter phosphorylation. Mol Psychiatry 19, 279–281 (2014). https://doi.org/10.1038/mp.2013.29

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