Common variant at 16p11.2 conferring risk of psychosis

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Abstract

Epidemiological and genetic data support the notion that schizophrenia and bipolar disorder share genetic risk factors. In our previous genome-wide association study, meta-analysis and follow-up (totaling as many as 18 206 cases and 42 536 controls), we identified four loci showing genome-wide significant association with schizophrenia. Here we consider a mixed schizophrenia and bipolar disorder (psychosis) phenotype (addition of 7469 bipolar disorder cases, 1535 schizophrenia cases, 333 other psychosis cases, 808 unaffected family members and 46 160 controls). Combined analysis reveals a novel variant at 16p11.2 showing genome-wide significant association (rs4583255[T]; odds ratio=1.08; P=6.6 × 10−11). The new variant is located within a 593-kb region that substantially increases risk of psychosis when duplicated. In line with the association of the duplication with reduced body mass index (BMI), rs4583255[T] is also associated with lower BMI (P=0.0039 in the public GIANT consortium data set; P=0.00047 in 22 651 additional Icelanders).

Introduction

Two structural variants, a balanced t(1;11) translocation interrupting DISC1 and a microdeletion at 22q11.2, were the first genetic polymorphisms to show compelling evidence of association with schizophrenia.1, 2 More recently, additional microdeletions and microduplications conferring risk of schizophrenia and, in some cases, bipolar disorder have been uncovered.3, 4, 5, 6, 7, 8, 9, 10 These copy number variants (CNVs) confer high-to-moderate relative risk, however, because they typically change copy number of multiple genes, and may also affect regulation of genes at their margins, they do not generally implicate individual genes.

Currently, common single nucleotide polymorphisms (SNPs) are convincing risk factors for schizophrenia and bipolar disorder, in addition to structural variants. Common alleles showing genome-wide significant association with at least one of the disorders have been found at more than 20 loci.11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 None of these regions are within structural polymorphisms previously shown to be susceptibility factors for schizophrenia or bipolar disorder. Nevertheless, first principles and data from other disorders predict the existence of common variants conferring risk through the same genes as rare structural alleles.30 The identification of common risk variants within CNV regions may aid in uncovering the causal gene or genes of a CNV, or help to elucidate other aspects of a CNV’s association with disease.

Two loci have been reported to harbor common alleles showing genome-wide significant association with both schizophrenia and bipolar disorder.13, 16, 23, 24 In addition, several common variants initially displaying genome-wide significant association with one of the disorders have been shown, in subsequent studies, to confer risk of the other.31, 32 Investigations considering schizophrenia and bipolar disorder as a single phenotype also support shared risk alleles,16, 19, 22 and an overlapping polygenetic component has been described by several studies.21, 28 These data are consistent with current epidemiological investigations, which predict shared genetic risk factors for schizophrenia and bipolar disorder.33

Previously, we carried out a schizophrenia genome-wide association (GWA) study, SGENE-plus, followed by meta-analysis of the top 1500 results with data from the International Schizophrenia Consortium (ISC) and the Molecular Genetics of Schizophrenia (MGS) group.15 Loci having P-values <1 × 10−4 (covered by 39 SNPs located in 33 genomic regions) were followed up in a data set of up to 10 260 schizophrenia cases and 23 500 controls.14 In this work, we broaden our phenotype of interest to psychosis (schizophrenia, bipolar disorder and related psychoses), examining the same group of follow-up SNPs in a data set augmented by 7469 bipolar disorder cases, 1535 schizophrenia cases, 333 other psychosis cases, 808 unaffected family members and 46 160 controls.

Materials and methods

Samples

The genome-wide typed (‘SGENE-plus’; 2663 cases and 13 498 controls) and meta-analysis (‘SGENE-plus+ISC+MGS’) samples (in total, 7946 cases and 19 036 controls) used here were identical to those used in our previous schizophrenia GWA study and meta-analysis.15 The primary psychosis follow-up samples employed consisted of follow-up samples from our previous GWA follow-up study (9246 schizophrenia cases and 22 356 controls),14 plus an additional 9337 psychosis cases (1535 schizophrenia, 7469 bipolar disorder and 333 related psychoses) and 46 968 controls/unaffected family members. The primary follow-up samples were genotyped or imputed for all follow-up markers. The secondary follow-up samples consisted of 1014 cases and 1144 controls from the Göttingen Research Association for Schizophrenia34, 35 study. These samples, which also had been used for secondary follow-up in our previous GWA follow-up study,14 were genotyped for SNPs that were genome-wide significant in the combined meta-analysis and primary follow-up samples. Table 1 summarizes the schizophrenia and psychosis data sets used in previous and current work, and Supplementary Table 1 includes details on the individual study groups. The autism samples (3773 cases, 16 103 controls, 4206 family members) derived from the Autism Genome Project, the Autism Genetic Resource Exchange and nine European study groups (Supplementary Table 2). Further information on ascertainment and diagnosis for the psychosis and autism samples is provided in the Supplementary Material.

Table 1 Schizophrenia and psychosis data sets used in previous and current work

Genotyping and association analysis

Genotyping was carried out using Illumina (San Diego, CA, USA) and Affymetrix genome-wide arrays (Santa Clara, CA, USA), Nanogen (San Diego, CA, USA) Centaurus assays, Taqman assays, the Sequenom MassArray iPLEX genotyping system (San Diego, CA, USA) and the Roche LightCycler480 system (Mannheim, Germany) (Supplementary Tables 1 and 2). Quality control and imputation were performed, by study group, as described in the Supplementary Methods. Case–control or family-based association analyses were carried out for each study group. For the case–control analyses, population stratification was controlled for using genomic control or principal components. Summary statistics from the various study groups were combined as described previously.15 Body mass index (BMI) measurements were adjusted for age and sex, and inverse standard normal transformed. Analysis was carried out by regressing the adjusted, transformed data on rs4583255[T] count.

Expression analysis

For the three brain data sets,36, 37, 38 expression levels were inverse normal transformed and regressed on the number of rs4583255-T alleles with gender, age at death, post-mortem interval, brain source, expression experiment batch, pH (Colantuoni et al.36 only), sample expression level based on the total number of transcripts detected (Webster et al.38 only) and Alzheimer’s disease patient status (Webster et al.38 only) as covariates. To incorporate data from different brain regions (Gibbs et al.37) or different probes (KCTD13 in Colantuoni et al.36) derived from the same individual, a mixed-effects model with individual as a random effect was used. Results from the three data sets were combined using inverse-variance weighted meta-analysis. The Dutch whole-blood data set included control samples from two studies.39, 40 Analysis was performed using linear regression in Plink41 taking age and gender as covariates. The Icelandic blood data set has been described previously,42 and analysis was carried out as detailed in that work.42

Results

We assembled a psychosis (schizophrenia, bipolar disorder and related psychoses) primary follow-up data set made up of 36 study groups containing a total of 18 583 cases, 68 516 controls and 808 unaffected family members (Supplementary Table 1). In each study group, allelic association analysis was carried out for 39 SNPs from 33 genomic regions (these SNPs covered P-values <1 × 10−4 in the SGENE-plus+ISC+MGS meta-analysis at r2=0.3). Results from the various study groups were combined using inverse-variance weighted meta-analysis.

At 31 of the 33 loci, odds ratios (ORs) in the psychosis follow-up group were in the same direction as in the discovery data set (SGENE-plus+ISC+MGS) (Supplementary Table 3). A similar pattern had been observed in the schizophrenia follow-up set—ORs were in the same direction at 30 of the 33 loci.14 These results indicate that the set of variants chosen for follow-up was enriched for risk alleles (P=7.0 × 10−7 for schizophrenia; P=6.5 × 10−8 for psychosis).

Next, we performed a joint analysis of the discovery and psychosis follow-up sets. To account for testing two phenotypes (schizophrenia and psychosis), the genome-wide significance threshold was set at P<(5 × 10−8)/2, or 2.5 × 10−8. Five SNPs, residing at three loci, exceeded this threshold (Supplementary Table 3). Two of the loci—the major histocompatibility complex region and 11q21.2 near NRGN—had been genome-wide significant in the previous schizophrenia analysis; a third locus, in TAOK2 at 16p11.2, was novel (Supplementary Table 3). Following the addition of data from a further 1014 schizophrenia cases and 1144 controls, the variant at the novel locus, rs4583255[T], was associated with psychosis with increased significance (OR=1.08, P=6.6 × 10−11, Table 2, Figure 1). rs4583255[T]’s association with psychosis fit the multiplicative model (P=0.42), and there was no evidence of OR heterogeneity (P=0.71; I2=0; Supplementary Table 4).

Table 2 Genome-wide significant association of rs4583255[T] with psychosis
Figure 1
figure1

Association results and structure of the 16p11.2 region. Bars on the x-axis indicate segmental duplications (brown) and recombination hotspots (pink). Association results are illustrated for SGENE-plus (black), SGENE-plus+MGS+ISC (green), SGENE-plus+MGS+ISC plus the primary psychosis follow-up (blue) and SGENE-plus+MGS+ISC plus the primary psychosis and secondary schizophrenia follow-up (red). RefSeq genes in the region are shown below the plot.

PowerPoint slide

In examination of the follow-up samples by diagnosis, the novel variant, rs4583255[T], showed significant association with both schizophrenia and bipolar disorder (P=0.0011 and 0.00026, respectively), with OR of 1.06 and 1.08, respectively (independent controls were used for the two analyses; see Supplementary Table 5). We also investigated association with bipolar disorder for variants that had shown genome-wide significant association with schizophrenia in our previous study.14 Following correction for eight tests, rs12807809[T], near NRGN, was significantly associated with bipolar disorder (P=0.0023) with an OR identical to that of the schizophrenia follow-up samples (OR=1.09). The remaining schizophrenia susceptibility variants did not show even nominally significant association with bipolar disorder—yet, OR confidence intervals for the two disorders overlapped for at least some variants at all loci (Supplementary Table 5).

Intriguingly, the newly identified SNP is located in a nearly 600-kb region that confers risk of schizophrenia and bipolar disorder when duplicated (Figure 1).5, 6, 28 Copy number gain of the region also is associated with autism,6, 43, 44, 45 reduced head circumference46, 47 and low BMI.47 We obtained large data sets to examine association of rs4583255[T] with both autism and BMI. Based on 3773 cases, 16 103 controls and 4206 unaffected family members from the Autism Genetic Resource Exchange, the Autism Genome Project and nine European study groups (Supplementary Table 2), we found no evidence of association with autism spectrum disorder (ASD), strict autism or multiplex ASD (ASD, OR=1.00, P=0.98; strict autism, OR=1.02, P=0.66; multiplex ASD, OR=1.07, P=0.22; Supplementary Table 6), although power to detect association at the OR found in the follow-up psychosis samples was modest (at a 0.05 significance level, power was about 57% for ASD, 42% for strict autism and 23% for multiplex ASD). In contrast, we found significant association of rs4583255[T] with lower BMI in the published GIANT consortium GWAS data set of 123 865 individuals48 (P=0.0039) and in 22 651 Icelanders who were not included in the GIANT study (P=0.00047).

Recently, a study examining the effect of altered expression of 16p11.2 CNV region genes on zebrafish head size identified KCTD13 as the major driver of head size change, with MAPK3 and MVP named as possible modifiers.49 These results motivated us to examine association of rs4583255[T] with expression of KCTD13, MAPK3 and MVP in human brain. Using data from three publicly available data sets with at least 50 European-ancestry adult brains each (total N=565),36, 37, 38 we found that rs4583255[T] was significantly associated with expression of MAPK3 (effect=0.12 s.d.; P=0.011), but not significantly associated with expression of KCTD13 or MVP (Supplementary Table 7). We also investigated association of rs4583255[T] with gene expression in blood using data sets from Iceland (N=972)42 and the Netherlands (N=437).39, 40 Consistent with the brain results, rs4583255[T] was significantly associated with higher expression of MAPK3 (for Iceland, P=9.4 × 10−15; for the Netherlands, P=0.014 for probe 3870601, and P=0.042 for probe 234040), but not significantly associated with expression of KCTD13 or MVP.

Discussion

In this study, we uncovered a novel variant at 16p11.2, rs4583255[T], showing genome-wide significant association with psychosis (OR=1.08; P=6.6 × 10−11). In follow-up samples, ORs were similar for schizophrenia and bipolar disorder (OR=1.06 and 1.08, respectively), and association was significant for both (P=0.0011 and 0.00026, respectively). Thus, rs4583255[T] is a compelling example of a genetic variant that confers risk across traditional diagnostic boundaries.

Among the variants that showed genome-wide significant association with schizophrenia in our previous study,14 only rs12807809[T] showed significant association with bipolar disorder in the current work. Nevertheless, OR confidence intervals for schizophrenia and bipolar disorder overlapped for most risk alleles. Very large data sets will be necessary to establish conclusively where these variants fall on the spectrum of conferring risk of one disorder, exclusively, to conferring equal risk of either.

To our knowledge, this is the first case in which a common risk allele showing genome-wide significant association with psychosis has turned out to be located within a CNV that had been previously associated with psychosis. Both copy number gain and loss of the 16p11.2 region are associated with multiple phenotypes. Duplication is associated with psychosis,5, 6, 28 both copy number gain and loss are associated with autism and developmental delay,6, 43, 44, 45 and duplication and deletion lead to reduction and enlargement, respectively, of head circumference and BMI.46, 47

In this work, we found that rs4583255[T] also confers risk of reduced BMI (P=0.0039 in GIANT; P=0.00047 in additional Icelanders). This result supports the suggestion, made previously,47 that the effects of duplication on psychosis and BMI have a single origin, presumably in the brain. We did not find evidence of association of rs4583255[T] with autism, although we were somewhat underpowered to detect an effect of the same size as in psychosis, especially, for sub-phenotypes.

We found that rs4583255[T] was associated with increased expression in adult brain and blood of MAPK3, one of the 16p11.2 genes identified as involved in causing head-circumference changes in zebrafish.49 Caution is required in interpretation of this result, however, as the significance in brain is not overwhelming and, furthermore, gene expression in the pre-adult brain may be most relevant for the development of psychosis. Data from only extremely small numbers of European-ancestry brains at pre-adult stages were available, precluding investigation of the association of rs4583255[T] with gene expression at these stages.

In conclusion, in this work, we broadened our phenotype of interest to psychosis, identifying a new common risk allele, rs4583255[T], with similar ORs for schizophrenia and bipolar disorder. The novel variant is located within a duplication previously associated with psychosis, and, in line with the duplication’s effects, also is associated with lower BMI. In the future, knowledge of this common variant association may prove useful to studies aimed at further understanding the mechanism through which the duplication exerts its effects on neurodevelopmental and anthropomorphic phenotypes.

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Acknowledgements

We would like to thank the subjects, their families and the recruitment centre staff. We would also like to acknowledge the help of Maria Dolores Moltó (Genetics Department, Valencia University, CIBERSAM), Eduardo Paz and Ramón Ramos-Ríos (Complexo Hospitalario de Santiago), and the contribution of Fundación Botín. This study makes use of seven external, publicly available data sets. First, it makes use of data generated by the Clinical Antipsychotic Trials of Intervention Effectiveness (CATIE) project whose principal investigators were Jeffrey A Lieberman, MD, T Scott Stroup, MD, MPH, and Joseph P McEvoy, MD. The CATIE trial was funded by a grant from the National Institute of Mental Health (N01 MH900001) along with MH074027 (PI PF Sullivan). Genotyping was funded by Eli Lilly and Company. Second, the GAIN/BiGs data sets used in this work were obtained from the database of Genotypes and Phenotypes (dbGaP) found at http://www.ncbi.nlm.nih.gov/gap through dbGaP accession number phs000017.v3.p1. Third, the study uses samples genotyped using the Ilumina 550K platform by the Pritzker Consortium, supported by the Pritzker Neuropsychiatric Disorders Research Fund L.L.C. The Pritzker Consortium includes scientists at the University of Michigan (H Akil and S J Watson, Site Directors, and Michael Boehnke, lead on bipolar genotyping effort); Stanford University (Rick Myers and Alan Schatzberg, Site Directors); the University of California at Davis (Ted Jones, Site Director); the University of California at Irvine (William Bunney, Site Director); and the Weill Medical College of Cornell University (Jack Barchas, Site Director). Fourth, the work uses data from the Systematic Treatment Enhancement Program for Bipolar Disorder (STEP-BD) project, led by Gary Sachs, MD, and coordinated by Massachusetts General Hospital in Boston, MA (NIMH grant number was 2N01MH080001–001). Fifth, this study makes use of data generated by the Wellcome Trust Case–Control Consortium. A full list of the investigators who contributed to the generation of the data is available from www.wtccc.org.uk. Funding for the project was provided by the Wellcome Trust under award 076113 and 085475. Sixth, we gratefully acknowledge the resources provided by the Autism Genetic Resource Exchange (AGRE) Consortium* and the participating AGRE families. The AGRE is a program of Autism Speaks and is supported, in part, by grant 1U24MH081810 from the National Institute of Mental Health to Clara M Lajonchere (PI). Seventh, the Autism Genome Project (AGP) data sets used for the analysis described in this manuscript were obtained from dbGaP at http://www.ncbi.nlm.nih.gov/gap through dbGaP accession number, phs000267.v1.p1. Submission of the data to dbGaP was provided by Dr Bernie Devlin on behalf of the AGP. Collection and submission of the data to dbGaP were supported by a grant from the Medical Research Council (G0601030) and the Wellcome Trust (075491/Z/04), Anthony P Monaco, PI, University of Oxford. This work was also supported by the European Union (grant numbers LSHM-CT-2006-037761 (Project SGENE), PIAP-GA-2008-218251 (Project PsychGene), HEALTH-F2-2009-223423 (Project PsychCNVs), HEALTH-F4-2009-242257 (Project ADAMS) and IMI-JU-NewMeds); the National Genome Research Network of the German Federal Ministry of Education and Research (BMBF) (grant numbers 01GS08144 (MooDS-Net) and 01GS08147 (NGFNplus)); the National Institute of Mental Health (R01 MH078075, and N01 MH900001, MH074027 to the Clinical Antipsychotic Trials of Intervention Effectiveness (CATIE) project); the Centre of Excellence for Complex Disease Genetics of the Academy of Finland (grant numbers 213506 and 129680); the Biocentrum Helsinki Foundation and Research Program for Molecular Medicine, Faculty of Medicine, University of Helsinki; the Stanley Medical Research Institute; the Danish Council for Strategic Research (grant number 2101-07-0059); H Lundbeck A/S; the Research Council of Norway (grant number 163070/V50); the Danish Medical Research Council; the South-East Norway Health Authority (grant number 2004-123); the Medical Research Council; Ministerio de Sanidad y Consumo, Spain (grant number PI081522 to JC); Xunta de Galicia (grant number 08CSA005208PR to A Carracedo); the Swedish Research Council; the Wellcome Trust (Wellcome Trust grants 085475/B/08/Z and 085475/Z/08/Z as part of the Wellcome Trust Case Control Consortium 2); the Max Planck Society; Saarland University (grant number T6 03 10 00–45 to CMF); the Netherlands Foundation for Brain Research (Hersenstichting) (grant number 2008(1).34 to M Poot); and Eli Lilly and Company (genotyping for CATIE and part of the TOP sample). For further acknowledgements, see the Supplementary Material.

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Correspondence to K Stefansson.

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Additional information

Supplementary Information accompanies the paper on the Molecular Psychiatry website

Genetic Risk and Outcome in Psychosis (GROUP)

René S. Kahn1, Don H. Linszen2, Jim van Os3, Durk Wiersma4, Richard Bruggeman4, Wiepke Cahn1, Lieuwe de Haan2, Lydia Krabbendam3 and Inez Myin-Germeys3

1Department of Psychiatry, Rudolf Magnus Institute of Neuroscience, University Medical Center Utrecht, Postbus 85060, Utrecht, The Netherlands

2Academic Medical Centre University of Amsterdam, Department of Psychiatry, Amsterdam, NL326 Groot-Amsterdam, The Netherlands

3Maastricht University Medical Centre, South Limburg Mental Health Research and Teaching Network, 6229 HX Maastricht, The Netherlands

4University Medical Center Groningen, Department of Psychiatry, University of Groningen, PO Box 30.001, 9700 RB Groningen, The Netherlands

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Supplementary information

Wellcome Trust Case Control Consortium 2

Wellcome Trust Case Control Consortium 2

Management committee

Peter Donnelly (Chair)1,2, Ines Barroso (Deputy Chair)3, Jenefer M Blackwell4,5, Elvira Bramon6, Matthew A Brown7, Juan P Casas8, Aiden Corvin9, Panos Deloukas3, Audrey Duncanson10, Janusz Jankowski11, Hugh S Markus12, Christopher G Mathew13, Colin NA Palmer14, Robert Plomin15, Anna Rautanen1, Stephen J Sawcer16, Richard C Trembath13, Ananth C Viswanathan17 and Nicholas W Wood18

Data and analysis group

Chris C A Spencer1, Gavin Band1, Céline Bellenguez1, Colin Freeman1, Garrett Hellenthal1, Eleni Giannoulatou1, Matti Pirinen1, Richard Pearson1, Amy Strange1, Zhan Su1, Damjan Vukcevic1 and Peter Donnelly1,2

DNA, genotyping, data QC and informatics group

Cordelia Langford3, Sarah E Hunt3, Sarah Edkins3, Rhian Gwilliam3, Hannah Blackburn3, Suzannah J Bumpstead3, Serge Dronov3, Matthew Gillman3, Emma Gray3, Naomi Hammond3, Alagurevathi Jayakumar3, Owen T McCann3, Jennifer Liddle3, Simon C Potter3, Radhi Ravindrarajah3, Michelle Ricketts3, Matthew Waller3, Paul Weston3, Sara Widaa3, Pamela Whittaker3, Ines Barroso3 and Panos Deloukas3.

Publications committee

Christopher G Mathew (Chair),13 Jenefer M Blackwell4,5, Matthew A Brown7, Aiden Corvin9 and Chris C A Spencer1

1Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, UK

2Department of Statistics, University of Oxford, Oxford OX1 3TG, UK

3Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK

4Telethon Institute for Child Health Research, Centre for Child Health Research, University of Western Australia, 100 Roberts Road, Subiaco, Western Australia 6008, Australia

5Cambridge Institute for Medical Research, University of Cambridge School of Clinical Medicine, Cambridge CB2 0XY, UK

6Department of Psychosis Studies, NIHR Biomedical Research Centre for Mental Health at the Institute of Psychiatry, King’s College London and The South London and Maudsley NHS Foundation Trust, Denmark Hill, London SE5 8AF, UK

7University of Queensland Diamantina Institute, Brisbane, Queensland, Australia

8Department of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK, and Department Epidemiology and Public Health, University College London WC1E 6BT, UK

9Neuropsychiatric Genetics Research Group, Institute of Molecular Medicine, Trinity College Dublin, Dublin, Ireland

10Molecular and Physiological Sciences, The Wellcome Trust, London NW1 2BE, UK

11Department of Oncology, Old Road Campus, University of Oxford, Oxford OX3 7DQ, UK, Digestive Diseases Centre, Leicester Royal Infirmary, Leicester LE7 7HH, UK, and Centre for Digestive Diseases, Queen Mary University of London, London E1 2AD, UK

12Clinical Neurosciences, St George’s University of London, London SW17 0RE, UK

13King’s College London Dept Medical and Molecular Genetics, King’s Health Partners, Guy’s Hospital, London SE1 9RT, UK

14Biomedical Research Centre, Ninewells Hospital and Medical School, Dundee DD1 9SY, UK

15King’s College London Social, Genetic and Developmental Psychiatry Centre, Institute of Psychiatry, Denmark Hill, London SE5 8AF, UK

16Department of Clinical Neurosciences, University of Cambridge Addenbrooke’s Hospital, Cambridge CB2 0QQ, UK

17NIHR Biomedical Research Centre for Ophthalmology, Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, London EC1V 2PD, UK

18Department of Molecular Neuroscience, Institute of Neurology, Queen Square, London WC1N 3BG, UK.

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Keywords

  • association
  • bipolar disorder
  • cross-disorder
  • schizophrenia
  • 16p11.2

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