Figure 7

From: Passive transfer of streptococcus-induced antibodies reproduces behavioral disturbances in a mouse model of pediatric autoimmune neuropsychiatric disorders associated with streptococcal infection

Figure 7

Immunoglobulin deposits in the brains of recipient mice passively injected with group A β-hemolytic streptococcus (GABHS) donor sera. Brains from naive SJL/J mice injected with either pooled GABHS or phosphate-buffered saline (PBS) donor sera, or immunoglobulin G (IgG)-depleted GABHS or PBS donor sera, were assessed for presence of IgG deposits using immunofluorescence techniques. Hippocampus (ad); periventricular area (eh). Recipient mice injected intravenously with nondepleted GABHS donor sera had evidence of IgG binding to dentate gyrus granule cell neurons in the hippocampus (a) and cells in the periventricular area (e), as revealed by anti-IgG staining (red signal). IgG staining in the hippocampal neurons (b) and in the periventricular area (f) was abrogated in mice injected with IgG-depleted GABHS donor sera. Anti-IgG immunoreactivity was absent in the brains of mice injected with nondepleted (c and g) or IgG-depleted PBS donor sera (d and h). Blue signal indicates nuclear counterstaining. gcl, granule cell layer of dentate gyrus; ml, molecular layer; Hi, hilus; LV, lateral ventricle. Scale bars, 40 μm.